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翌圣生物科技（上海）股份有限

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Enzyme Commission （EC） Number: 1.1.1.49
CAS Number: 9001-40-5
Molecular Weight:
128 kDa （gel filtration）9
103.7 kDa for the dimer （equilibrium sedimentation）1
Approximay 50 kDa for the monomer

詳細(xì)介紹

Synonym:

G-6-P-DH; Glucose-6-phosphate Dehydrogenase;葡萄糖-6-磷酸脫氫酶;6-磷酸葡萄糖脫氫酶

Product Description

Enzyme Commission （EC） Number: 1.1.1.49

CAS Number: 9001-40-5

Molecular Weight:

128 kDa （gel filtration）9

103.7 kDa for the dimer （equilibrium sedimentation）1

Approximay 50 kDa for the monomer （denaturing gel electrophoresis）2

ëmax: 280.5 nm1

Extinction Coefficient: E0.1% = 1.15 （280.5 nm, 0.09 M

Tris-HCl, pH 7.2）1

Glucose-6-Phophate Dehydrogenase （G-6-PDH） consists of two subunits of equivalent molecular weight.The amino acid sequence of the monomer has been published

G-6-PDH has been utilized in assays for nicotinamideadenine dinucleotide and tissue pyridine nucleotides

The KM value for NAD+ as a substrate is approximay 1.8 times better than that for NADP+. At pH 7. in Tris buffer, KM = 5.3 x 10-4 M for glucose-6-phosphate and KM = 0.99 x 10-4 M for NADP+.6 Binding constants have been reported for the native and pyridoxalmodified

enzyme. A lysine is in the active site （modified by pyridoxal phosphate）.

G-6-PDH can be reactivated from urea-denatured solutions.

Glucose 6-phosphate dehydrogenase is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH oxidizes glucose-6-phosphate in the presence of NADP+ to yield 6-phosphogluconate. Polyacrylamide gel electrophoresis, activity staining, and anti-yeast G-6-PDH antibody immunoblotting studies have indicated that G-6-PDH is a glycoprotein.

This product is approximay 25% protein; the remainder is the buffer from which it is lyophilized. The product is lyophilized in the presence of a neutral, colloidal stabilizer, Tris buffer, and a trace of magnesium chloride. The stabilizer added to this product is an inert neutral polymer, which is not expected to interfere in a biological assay. It protects the product through the lyophilization process. A DEAE ion exchange column can be used with a buffer

of approximay pH 7.3 to remove this stabilizer. Product No. G 5760 is an ammonium sulfate

suspension of the enzyme and does not contain this stabilizer.

Precautions and Disclaimer

For Laboratory Use Only. Not for drug, household or other uses.

Preparation Instructions

The product can be dissolved at 1 mg/ml in 5 mM glycine buffer, pH 8, （with or without 0.1% BSA） or in deionized water at 1 mg/ml （buffer is preferable）.

Storage/Stability

Solutions dissolved at 1 mg/ml in 5 mM glycine buffer, pH 8, （with or without 0.1% BSA） or in deionized water （1 mg/ml） can be aliquoted and stored frozen for approximay 2 months. It is best to subject the aliquot to no more than one freeze/thaw cycle. For storage in the refrigerator, use an ammonium sulfate suspension of this enzyme （Product No. G 5760）.

Ordering Information

貨號	名稱	產(chǎn)地	規(guī)格	報(bào)價(jià)	*（元）	庫存
G5885	G6PD 葡萄糖-6-磷酸脫氫酶	Sigma	1000U	2063	980	是
G5885	G6PD 葡萄糖-6-磷酸脫氫酶	Sigma	2000U	2995	1764	是
G5885	G6PD 葡萄糖-6-磷酸脫氫酶	Sigma	10KU	10325	6195	是

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