咨詢：  （重慶） （貴陽(yáng)）

Anti-ZO1 tight junction protein antibody (ab59720)抗ZO1緊密連接蛋白抗體產(chǎn)品信息：

• Product name：Anti-ZO1 tight junction protein antibodySee all ZO1 tight junction protein primary antibodies ...
• Description

  Rabbit polyclonal to ZO1 tight junction protein

• Specificityab59720 recognises both ZO1 tight junction alpha-minus and alpha-plus isoforms. The protein is abundant in endothelial cells and the highly specialized epithelial junctions. Although we have an image for IHC-Fr, customers report variable results in this application. If you would like to use in IHC-Fr then we suggest using ethanol instead of acetone. Although we have references for IHC-P, customers report variable results in this application. IHC-Fr and IHC-P are therefore not covered by our Abpromise guarantee.
• Tested applicationsICC/IF, Flow Cyt, IHC (PFA fixed), WB, IHC-P more details
• Species reactivity

  Reacts with: Mouse, Chicken, Dog, Human, Pig

• Immunogen

  Corresponding to amino acids 432-1150 of Human ZO1 tight junction protein

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 0.1% BSA, PBS

• Concentration 50 µg at 0.1 mg/ml

• Purification notes0.2µm filtered.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Tags & Cell Markers
  • Subcellular Markers
  • Plasma Membrane

Applications

Our Abpromise guarantee covers the use of ab59720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

• FunctionThe N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
• Tissue specificityThe alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
• Sequence similaritiesBelongs to the MAGUK family.
  Contains 1 guanylate kinase-like domain.
  Contains 3 PDZ (DHR) domains.
  Contains 1 SH3 domain.
  Contains 1 ZU5 domain.
• DomainThe second PDZ domain mediates interaction with GJA12.
• Post-translational
  modificationsPhosphorylated. Dephosphorylated by PTPRJ.
• Cellular localizationCell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
• Information by UniProt

• Database links

  • Entrez Gene: 7082 Human
  • Entrez Gene: 21872 Mouse
  • Omim: 601009 Human
  • SwissProt: Q07157 Human
  • SwissProt: P39447 Mouse
  • Unigene: 510833 Human
  • Unigene: 4342 Mouse

• Alternative names

  • Tight junction protein 1 antibody
  • Tight junction protein ZO-1 antibody
  • Tight junction protein ZO1 antibody

Anti-ZO1 tight junction protein antibody images

• Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab59720)

  ab59720 staining ZO1 in Caco-2 cells treated with (±)-palmitoylcarnitine chloride (ab141122), by ICC/IF. Membranar ZO1 expression loss correlates with increased concentration of (±)-palmitoylcarnitine chloride, as described in literature.
  The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab141122 ((±)-palmitoylcarnitine chloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab59720 (0.5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

• 

  Immunocytochemistry/ Immunofluorescence - ZO1 tight junction protein antibody (ab59720)

  ICC/IF image of ab59720 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59720, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab59720)This image is courtesy of an anonymous Abreview

  Immunofluorescence analysis of HUVEC cells, staining ZO1 tight junction protein with ab59720.
  Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab59720)Image from Kokkinopoulos I et al., PLoS One. 2011 Apr 22;6(4):e18921. Fig 6.; doi:10.1371/journal.pone.0018921; April 22, 2011, PLoS ONE 6(4): e18921.

  Immunofluorescence analysis of murine peripheral retinal pigmented epithelium (RPE) cells, staining ZO1 tight junction protein with ab59720.
  
  Cells were fixed with 4% paraformaldehyde for 10 minutes at room temperature. Cells were blocked in 10% BSA and 0.5% Triton-X 100 for 2 hours at room temperature before being incubated with primary antibody (1/100) in blocking solution overnight at room temperature. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody.

公司介紹：

    Abcam位于英國(guó)的劍橋科學(xué)園，成立于1998年，專(zhuān)門(mén)生產(chǎn)和分銷(xiāo)研究型抗體。主要?jiǎng)?chuàng)立人Jonathan Milner和實(shí)驗(yàn)室同事構(gòu)思成立一間了解科研人員需要的網(wǎng)上抗體公司，理念是Abcam銷(xiāo)售質(zhì)量高的抗體，并提供zui全面、誠(chéng)實(shí)、時(shí)新的說(shuō)明書(shū)，快速的送貨服務(wù)，及高效率的顧客和服務(wù)。我們的在線目錄(www.abcam.cn) 已有差不多100,000種抗體和試劑，并不斷添加，供應(yīng)予百多個(gè)國(guó)家。Abcam于2005年11月在倫敦證券交易所上市，在美國(guó)馬薩諸塞州、日本東京及香港均設(shè)有分公司。

    Abcam 一直致力加強(qiáng)產(chǎn)品線，為使研究員更容易找到蛋白質(zhì)研究試劑產(chǎn)品，在2011并購(gòu)了美國(guó)的MitoSciences公司，加強(qiáng)了免疫分析方面的產(chǎn)品供應(yīng)；同年也并購(gòu)了英國(guó)的Ascent Scientific 公司，開(kāi)展了生化試劑的供應(yīng)。在2012年，Abcam并購(gòu)了美國(guó)的Epitomics 公司，成為一家有的RabMAbs® 供應(yīng)商。

    Abcam 的 目標(biāo)是給世界上的抗體建立zui大的在線目錄，為各地科學(xué)家提供*產(chǎn)品，成為各國(guó)科學(xué)界的重要伙伴。我們?yōu)樗挟a(chǎn)品提供來(lái)使客戶獲得預(yù)期的結(jié) 果。為提供高質(zhì)量的抗體來(lái)指向盡可能多的靶蛋白，我們盡所能在盡可能多的應(yīng)用和物種中檢測(cè)每種抗體。我們相信誠(chéng)信才是上策，在我們的上會(huì)發(fā)布有關(guān)我們 每一種產(chǎn)品的盡可能多的信息。

更多產(chǎn)品：

Abcamab1132Mouse monoclonal [1718] to Thyroid Hormone Receptor100ul3996Thyroid Hormone Receptor抗體一抗

Abcamab11320Rabbit polyclonal to gamma Tubulin - Centrosome Marker100ul3996gamma Tubulin抗體一抗

Abcamab11321Rabbit polyclonal to gamma Tubulin - Centrosome Marker100ul3996gamma Tubulin抗體一抗

Abcamab11322Mouse monoclonal [TUB-11] to epsilon Tubulin100ul3996epsilon Tubulin抗體一抗

Abcamab11323Mouse monoclonal [6-11B-1] to Tubulin （acetyl K40）100ul3996Tubulin抗體一抗

Abcamab11324Mouse monoclonal [B3] to Tubulin - Polyglutamylated100ul4274Tubulin抗體一抗

Abcamab11325Mouse monoclonal [TUB-1A2] to Tubulin100ul3996Tubulin抗體一抗

Abcamab11326Mouse monoclonal [100/2] to alpha Adaptin100ul3996alpha Adaptin抗體一抗

Abcamab11327Mouse monoclonal [100/1] to AP1+2 beta1 00ul3996beta 1/2 Adaptin抗體一抗

Abcamab11328Mouse monoclonal [100/3] to gamma Adaptin100ul3996gamma Adaptin抗體一抗

Abcam  ab12964Rabbit polyclonal to Corticotropin Releasing Factor Receptor 250ug5029Corticotropin Releasing Factor Receptor 2hystemcell一抗

Abcam  ab1297Rabbit polyclonal to SMC3 （Agarose）50ug3996SMC3hystemcell一抗

Abcam  ab12974Rabbit polyclonal to EMR350ug3996EMR3hystemcell一抗

Abcam  ab12977Rabbit polyclonal to Endothelin A Receptor50ug4773Endothelin A Receptorhystemcell一抗

Abcam  ab1298Mouse monoclonal [mAbcam 1298] to Histone H3 （di methyl K9）100ug3996Histone H3hystemcell一抗

Abcam  ab12986Rabbit polyclonal to Estrogen Related Receptor beta50ug3996Estrogen Related Receptor betahystemcell一抗

Abcam  ab12989Rabbit polyclonal to ETL50ug3996ETLhystemcell一抗

Abcam  ab1299Mouse monoclonal [NN 2C10/1] to GM130 - Golgi Marker100ug3996GM130hystemcell一抗

Abcam  ab12990Rabbit polyclonal to FPR150ug4940FPR1hystemcell一抗

Abcam  ab12994Rabbit polyclonal to Frizzled 650ug4940Frizzled 6hystemcell一抗

Abcam  ab13001Rabbit polyclonal to GALR150ug3996GALR1hystemcell一抗

Abcam  ab13003Rabbit polyclonal to GALR350ug4940GALR3hystemcell一抗

Abcam  ab13004Rabbit polyclonal to Gastric Inhibitory Polypeptide Receptor50ug4607Gastric Inhibitory Polypeptide Receptorhystemcell一抗

Abcam  ab13008Aurora A peptide100ug2010Aurora A蛋白/多肽多肽

Abcam  ab1301Goat polyclonal to LASP1100ug3996LASP1hystemcell一抗

Abcam  ab13011Rabbit polyclonal to GnRHR50ug4940GnRHRhystemcell一抗

Abcam  ab13015Rabbit polyclonal to HRH325ug3996HRH3hystemcell一抗

Abcam  ab13016Rabbit polyclonal to HUNK50ug3996HUNKhystemcell一抗

Abcam  ab1302Goat polyclonal to CrkL100ug3996CrkLhystemcell一抗

Abcam  ab13022Rabbit polyclonal to LPHN350ug4940LPHN3hystemcell一抗

Abcam  ab13025Rabbit polyclonal to GPR10850ug4940GPR108hystemcell一抗

Abcam  ab13026Rabbit polyclonal to EDG250ug3996EDG2hystemcell一抗

Abcam  ab13027Rabbit polyclonal to EDG225ug3996EDG2hystemcell一抗

Abcam  ab13028Rabbit polyclonal to EDG450ug3996EDG4hystemcell一抗

Abcam  ab13033Rabbit polyclonal to MC1 Receptor50ug4940MC1 Receptorhystemcell一抗

Abcam  ab13034Rabbit polyclonal to MC4 Receptor50ug3996MC4 Receptorhystemcell一抗

Abcam  ab13037Rabbit polyclonal to Metabotropic Glutamate Receptor 150ug3996Metabotropic Glutamate Receptor 1hystemcell一抗

Abcam  ab1304Mouse monoclonal [10] to Vascular Endothelium100ug3996Vascular Endotheliumhystemcell一抗

Abcam  ab13040Rabbit polyclonal to Metabotropic Glutamate Receptor 250ug3996Metabotropic Glutamate Receptor 2hystemcell一抗

Abcam  ab13041Rabbit polyclonal to Metabotropic Glutamate Receptor 350ug3996Metabotropic Glutamate Receptor 3hystemcell一抗

Abcam  ab13042Rabbit polyclonal to Metabotropic Glutamate Receptor 750ug3996Metabotropic Glutamate Receptor 7hystemcell一抗

Abcam  ab13046Mouse monoclonal [CYT3-10] to Cytohesin 3100ul3996Cytohesin 3hystemcell一抗

Abcam  ab13048Mouse monoclonal [23A8] to RAC50ug3996RAChystemcell一抗

Abcam  ab13049Mouse monoclonal [ARAN1] to Ran50ul3996Ranhystemcell一抗

Abcam  ab1305Goat polyclonal to Smad2100ug3996Smad2hystemcell一抗

Abcam  ab13051Rabbit polyclonal to Oxytocin Receptor50ug5029Oxytocin Receptorhystemcell一抗

Abcam  ab13057Mouse monoclonal [B4F8] to RASGAP50ug3996RASGAPhystemcell一抗

Abcam  ab13058Mouse monoclonal [24E4] to REM100ug3885REMhystemcell一抗

Abcam  ab13059Mouse monoclonal [11D1] to proBNP200ug3996proBNPhystemcell一抗

Abcam  ab13065Rabbit polyclonal to Muscarinic Acetylcholine Receptor M450ug3996Muscarinic Acetylcholine Receptor M4hystemcell一抗

Abcam  ab13066Rabbit polyclonal to NPFF1 Receptor50ug3996NPFF1 Receptorhystemcell一抗

Abcam  ab1307Goat polyclonal to FOXP2100ug3996FOXP2hystemcell一抗

Abcam  ab13071Rabbit polyclonal to Olfactory Receptor LS18974450ug4940Olfactory Receptor LS189744hystemcell一抗

Abcam  ab13073Rabbit polyclonal to OR2H350ug3996OR2H3hystemcell一抗

Abcam  ab13074Rabbit polyclonal to Mu Opioid Receptor50ug3996Mu Opioid Receptorhystemcell一抗

Abcam  ab13078Rabbit polyclonal to Parathyroid Hormone Receptor 150ug3996PTHR 1hystemcell一抗

Abcam  ab1308Goat polyclonal to ARFGAP3100ug3996ARFGAP3hystemcell一抗

Abcamab11329Mouse monoclonal [AP180-I] to AP180100ul3996AP18抗體一抗

Abcamab1133Rabbit polyclonal to GABARAP100ul3996GABARAP抗體一抗

Abcam  ab11463Mouse monoclonal [BRIC 203] to Blood Group Kell Antigen100ug3996Blood Group Kell Antigenhystemcell一抗

Abcam  ab11464Rat monoclonal [HEK/1/85a] to CCR5100ug3996CCR5hystemcell一抗

Abcam  ab11465Rat monoclonal [HEK/1/85a] to CCR5 （Biotin）50ug3996CCR5hystemcell一抗

Abcam  ab11466Rat monoclonal [HEK/1/85a] to CCR5 （FITC）100ug4940CCR5hystemcell一抗

Abcam  ab11467Hamster monoclonal [ GL3 ] to TCR gamma+TCR delta （FITC）100ug3996TCR g/dhystemcell一抗

Abcam  ab1147Rabbit polyclonal to CSEN100ul3996DREAMhystemcell一抗

Abcam  ab11470Mouse monoclonal [P2W7] to Integrin alpha V50ug3996Integrin alpha Vhystemcell一抗

Abcam  ab11472Rabbit polyclonal to LIN7100ul3996LIN7hystemcell一抗

Abcam  ab11473Rabbit polyclonal to MAGI150ul3996MAGI1hystemcell一抗

Abcam  ab11476Sheep polyclonal to MAGI350ug3996MAGI3hystemcell一抗

Abcam  ab11477Histone H3 peptide - phospho S10100ug2010Histone H3蛋白/多肽多肽

Abcam  ab11478Hamster monoclonal [GL3] to TCR gamma+TCR delta （Phycoerythrin）250ul3996TCR g/dhystemcell一抗

Abcam  ab1148Rabbit polyclonal to ESE1100ul3996ESE1hystemcell一抗

Abcam  ab11480Rat monoclonal [KT50] to TCR V alpha 8 （Biotin）500ul5029TCR V alpha 8hystemcell一抗

Abcam  ab11482Rat monoclonal [TR310] to TCR V beta 7100ug3996TCR V beta 7hystemcell一抗

Abcam  ab11483Rat monoclonal [TR310] to TCR V beta 7 （FITC）100ug3996TCR V beta 7hystemcell一抗

Abcam  ab11484Mouse monoclonal [13B9] to Calcitonin （HRP）200ug3996Calcitoninhystemcell一抗

Abcam  ab11485Rat monoclonal [TR310] to TCR V beta 7 （Phycoerythrin）250ul3996TCR V beta 7hystemcell一抗

Abcam  ab11488Rat monoclonal [KJ16] to TCR V beta 8.1+ 8.2100ug3996TCR V beta 8.1+8.2hystemcell一抗

Abcam  ab1149Rabbit polyclonal to IKAP100ul3996IKAPhystemcell一抗

Abcam  ab11490Mouse monoclonal [18B7] to Calcitonin100ug3996Catacalcinhystemcell一抗

Abcam  ab11493Mouse monoclonal [16B5 ] to Calcitonin250ug3996Calcitoninhystemcell一抗

Abcam  ab11495Rat monoclonal [KT10b] to TCR V beta 10b2ml3996TCR V beta 1bhystemcell一抗

Abcam  ab11497Mouse monoclonal [24B2 ] to Calcitonin500ug3885Calcitoninhystemcell一抗

Abcam  ab11499Rat monoclonal [KT10b] to TCR V beta 10b （Biotin）500ul3996TCR V beta 1bhystemcell一抗

Abcam  ab1150Rabbit polyclonal to IKAP100ul3996IKAPhystemcell一抗

Abcam  ab11501Rabbit polyclonal to Nephronophthisis50ug3996Nephronophthisishystemcell一抗

Abcam  ab11503Goat polyclonal to Osteopontin25ug3996Osteopontinhystemcell一抗

Abcam  ab11504Rat monoclonal [KT12] to TCR V beta 12  2ml3996TCR V beta 12hystemcell一抗

Abcam  ab11505Mouse monoclonal [G3] to CD3 zeta （FITC）500ul3996TCR zeta chainhystemcell一抗

Abcam  ab11506Mouse monoclonal [15F11] to gamma Catenin100ul3996gamma Cateninhystemcell一抗

Abcam  ab11507Rabbit polyclonal to delta 1 Catenin50ul3996delta 1 Cateninhystemcell一抗

Abcam  ab11508Mouse monoclonal [6H11] to delta 1 Catenin50ul3996delta 1 Cateninhystemcell一抗

Abcam  ab11509Mouse monoclonal [SLP-32] to MAGI350ul3996SLIPRhystemcell一抗

Abcam  ab1151Rabbit polyclonal to IKK gamma100ul3996IKK gammahystemcell一抗

更多詳情，請(qǐng)：     

重慶市華雅干細(xì)胞技術(shù)有限公司