Recombinant B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF)  

B-Raf原癌基因絲氨酸/蘇氨酸蛋白激酶(BRAF)重組蛋白

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Ile457~Leu717

Tags: N-terminal His Tag

Subcellular Location: Membrane, Nucleus, Cytoplasm

Purity: > 95%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl, 5%Trehalose.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.5

Predicted Molecular Mass: 33.4kDa

Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

             Store at 2-8oC for one month.

             Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

[  IDENTIFICATION  ]

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.

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