Recombinant F-Box Protein 32 (FBXO32) 

F-框蛋白32(FBXO32)重組蛋白

[ PROPERTIES ]

Residues: Val155~Phe471

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q9UK99

Host: E. coli

Subcellular Location: Nucleus.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 4.6

Predicted Molecular Mass: 40.2kDa

Accurate Molecular Mass: 44kDa as determined by SDS-PAGE reducingconditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins fromthe samegene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile ddH₂O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

             Store at 2-8oC for one month.

             Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The sequence of the target protein is listed below.

VVPGLL GSMALSNHYR SEDLLDVDTA AGGFQQRQGL KYCLPLTFCI HTGLSQYIAVEAAEGRNKNE VFYQCPDQMA RNPAAIDMFI IGATFTDWFT SYVKNVVSGGFPIIRDQIFRYVHDPECVAT TGDITVSVST SFLPELSSVH PPHYFFTYRI RIEMSKDALP EKACQLDSRYWRITNAKGDV EEVQGPGVVG EFPIISPGRV YEYTSCTTFS TTSGYMEGYY TFHFLYFKDKIFNVAIPRFH MACPTFRVSI ARLEMGPDEY EEMEEEEEEE EEEDEDDDSA DMDESDEDDEEERRRRVFDV PIRRRRCSRL F

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