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          ProPac SCX and WCX Protein Column

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          • ProPac SCX and WCX Protein Column
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          ProPac SCX and WCX Protein ColumnNew! The Thermo Scientific ProPac™ SCX column line has expanded to include the next generation ProPac SCX-20 column. This column provides unequalled high resolution of

          詳細介紹

          Strong and Weak Cation-Exchange Columns for High-Resolution Separation of Proteins and MAbs

          New! The Thermo Scientific ProPac SCX column line has expanded to include the next generation ProPac SCX-20 column. This column provides unequalled high resolution of protein variants.

          Please also see the Thermo Scientific MAbPac SCX-10 column, a strong cation-exchange column designed specifically for high-resolution, high-efficiency analysis of monoclonal antibodies (MAbs) and associated variants. This column is complimentary to the industry-leading ProPac WCX-10 column for monoclonal antibody variant analysis. The MAbPac SCX-10 offers an alternative selectivity and provides higher resolution and efficiency for variant analysis of most monoclonal antibody samples than the ProPac WCX-10 column.

          ProPac SCX-10 and WCX-10 strong and weak cation-exchange columns are based on a nonporous core particle that results in high-efficiency peaks and high-resolution separations of protein variants. A hydrophilic layer prevents unwanted secondary interactions. A grafted cation-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.

          • Characterization and quality control assessment of monoclonal antibodies and other proteins

          • Unequalled resolution

          • High-resolution separations with excellent sample recovery

          • Unmatched column-to-column and lot-to-lot reproducibility

          • Useful for characterization of related protein variants (e.g., deamidation and MAb lysine truncation variants)

          • Highly-efficient peaks with excellent sample recovery

          • Available in different dimensions and formats for flexibility in method development

          The ProPac SCX-10 is a strong cation-exchange column with sulfonate functional groups, and the ProPac WCX-10 column has a carboxylate functional group. Both are based on 10 µm nonporous, polymeric beads and coated with a proprietary hydrophilic layer.

          All ProPac columns are manufactured and tested under the strictest specifications, resulting in unmatched column-to-column and lot-to-lot reproducibility. These columns are available as lot-select packages containing three columns from a single lot or three columns from three different lots, to assist quality control scientists in method validation.

          ProPac SCX-10 Strong Cation-Exchange Column for the High-Resolution Separation of Protein Isoforms  Specifications
          Maximum operating pressure3000 psi (21 MPa)
          Maximum operating temperature60 °C
          Recommended operating temperature:<40 °C
          Typical operating conditionsUV detection at 1 mL/min
          Recommended flow rate: 1 mL/min
          phase compatibilitypH 2–12; 80% compatible with acetonitrile, acetone, methanol.
          Compatible with nonionic and zwitterionic detergents.
          Incompatible with cationic detergents.
          Resin composition10-µm-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the sulfonate functional groups.
          Anion-exchange capacity< 100 µg protein on-column, depending upon the protein
          (4 × 250 mm analytical column)
          Column constructionPEEK with 10-32 threaded ferrule-style end fittings.
          All components are nonmetallic.

           

            ProPac WCX-10 Weak Cation-Exchange Column for the High-Resolution Separation of Protein Isoforms  Specifications
            Resin composition10-µm-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the carboxylate functional groups.
            Anion-exchange capacity< 100 µg protein on-column, depending upon the protein
            (4 × 250 mm analytical column)
            Column constructionPEEK with 10-32 threaded ferrule-style end fittings.
            All components are nonmetallic.
            Maximum operating pressure3000 psi (21 MPa)
            Maximum operating temperature60 °C
            Recommended operating temperature:<40 °C
            Typical operating conditionsUV detection at 1 mL/min
            Recommended flow rate: 1 mL/min
            phase compatibilitypH 2–12; 80% compatible with acetonitrile and acetone.
            Compatible with nonionic and zwitterionic detergents.
            Incompatible with cationic detergents.
             

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