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Fast, Sensitive Mono- and Disaccharide Analysis
The Thermo Scientific Dionex CarboPac™ PA20 column line has been expanded to include a new 0.4 × 150 mm capillary column and a new Dionex CarboPac PA20 Fast Sialic Acid column.
As with all CarboPac PA20 columns, the new capillary format provides high-resolution separations of mono- and disaccharides with no need for derivatization. The Dionex CarboPac PA20, 0.4 × 150 mm format offers the additional advantages of higher mass sensitivity, lower sample amount requirements, and significantly lower solvent use.
The new Dionex CarboPac PA20 Fast Sialic Acid column is ideally suited for the fast analysis of sialic acids in glycoprotein acid hydrolyzates. The Dionex CarboPac PA20 Fast Sialic Acid column accelerates sialic acid determination allowing sample analysis in less than five minutes. The Dionex CarboPac PA20 Fast Sialic Acid column is packed with the same hydrophobic, polymeric, pellicular anion exchange resin as the Dionex CarboPac PA20 column, but in a 3 x 30 mm column format.
The Dionex CarboPac PA20 is a strong anion-exchange column that provides high-resolution separations of mono- and disaccharides with no need for derivatization. This column provides baseline resolution between the six monosaccharides commonly found in mammalian glycoproteins over a wide range of eluent concentrations. The Dionex CarboPac PA20 provides slightly different selectivity from either the Dionex CarboPac PA1 or Dionex CarboPac PA10, making it an attractive choice for separating difficult peak pairs.
The column of choice for high-sensitivity monosaccharide analyses
Highly efficient separations with good resolution between neighboring peaks
Fast analysis—less than 10 min
Optimized resolution of glucosamine/galactose and glucose/mannose peak pairs
Lower eluent consumption
Does not require derivatization
The Dionex CarboPac PA20 consists of 6.5 µm-diameter nonporous beads covered with a fine latex of functionalized Thermo Scientific Dionex MicroBead™ resin. This pellicular resin structure permits excellent mass transfer, resulting in high-resolution chromatography and rapid re-equilibration. The performance of each column is verified with a Thermo Scientific Dionex MonoStandard™.
| Dionex CarboPac PA20 Column for Fast Monosaccharide Analysis Specifications | |
|---|---|
| Dimensions | Dionex CarboPac™ PA20 Analytical Column: 3 × 150 mm Dionex CarboPac PA20 Capillary Column: 0.4 × 150 mm Dionex CarboPac PA20 Fast Sialic Acid Column: 3 × 30 mm Dionex CarboPac PA20 Guard Column: 3 × 30 mm |
| Maximum operating pressure | 3000 psi (21 MPa) |
| Temperature range | 4–60 °C Recommended operating temperature: 30 °C |
| Typical operating conditions | Test procedure: Baseline resolution of fucose, galactosamine, glucosamine, galactose, glucose, mannose. Dionex CarboPac PA20 Analytical Column recommended flow rate: 0.5 mL/min Dionex CarboPac PA20 Capillary Column recommended flow rate: 8 µL/min Dionex CarboPac PA20 Fast Sialic Acid Column recommended flow rate: 0.5 mL/min |
| phase compatibility | pH 0–14; * compatible with common HPLC solvents and * water. Ionic form eluents: sodium hydroxide, sodium acetate. |
| Resin composition | 6.5 µm-diameter substrate (ethylvinylbenzene 55% crosslinked with divinylbenzene) agglomerated with 130 nm MicroBead™ difunctional quaternary ammonium ion (5% crosslinked). |
| Anion-exchange capacity | Approximay 65 µeq/column (3 × 150 mm analytical column) Approximay 1.16 µeq/column (0.4 × 150 mm capillary column) Approximay 13 µeq/column (0.3 x 30 mm Fast Sialic Acid column) |
| Column construction | PEEK™ with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |