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For High-Resolution Separations of Free Amino Acids by AAA-Direct
The AminoPac® PA10 column is packed with a hydrophobic, polymeric, pellicular, anion-exchange resin that is stable over the range of pH 0–14. The unique pH stability allows the use of eluents that are conducive to anodic oxidation of amino acids at gold electrodes. This column is recommended for use with the AAA-Direct™ Amino Acid Analyzer, allowing direct detection of primary and secondary amino acids by IPAD, with no need for pre- or postcolumn derivatization.
Derivatization not required
Carbohydrates and amino acids separated and detected simultaneously
Mid-femtomole to low-picomole detection limits
Compatible with all commonly used hydrolysis procedures
Can be configured for on-line analysis
AAA-Direct is a technique that eliminates the need for any form of derivatization, and is therefore compatible with on-line monitoring of cell cultures and fermentation broths.
The AminoPac PA10 column is optimized for separations of free amino acids by AAA-Direct. Vitamins, amino sugars, and carbohydrates can be detected simultaneously with amino acids. Additional capabilities include separation of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids, such as cysteic acid, methionine sulfone, and methionine sulfoxide.
| AminoPac PA10 Column for Amino Acid Analysis by AAA-Direct Specifications | |
|---|---|
| Recommended operating temperature | 30 °C |
| Typical operating conditions | 0.25 mL/min gradient Recommended flow rate: 0.25 mL/min |
| phase compatibility | pH 0–14; * compatible with common organic solvents. Dionex sodium acetate ionic form eluents only. |
| Resin composition | 8.5-µm-diameter microporous (<10 Å) bead (55% cross-linked) agglomerated with an 80-nm microbead alkyl ammonium functionalized latex (1% cross-linked). |
| Anion-exchange capacity | 60 µeq/column (2 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |
| Dimensions | AminoPac PA10 Analytical Column: 2 × 250 mm AminoPac PA10 Guard Column: 2 × 50 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |