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Monolithic Columns for Fast, High-Resolution Protein and Peptide Analysis
PepSwift monolithic columns are specially designed for fast separation and identification of proteins and peptides using nano and capillary LC coupled to MS. PepSwift columns are based on a polystyrene divinylbenzene copolymer bed. The bed structure of the column offers a high-quality alternative to traditional microparticulate sorbents, providing important advantages for high-sensitivity proteomics applications.
Polymeric monolithic stationary phases
High-speed protein/peptide separations (< 10 min)
High separation efficiency (> 200.000 plates/m)
High column-to-column reproducibility
High sensitivity in LC/MS
Superior lifetime
Available in 100 μm, 200 μm and 500 μm i.d
Available in 5 and 25 cm length (only 100 and 200 µm i.d.)
nanoViper fitting
PepSwift Trap columns can be used for preconcentration and desalting of samples consisting of peptides and proteins without negative impact on the chromatographic performance or recovery of the compounds. Various ion-pairing agents can be used in the loading solvent and/or phases to change the selectivity of the separation or improve the trapping efficiency.
| PepSwift and ProSwift Capillary Monolithic Reversed-Phase Columns Specifications | |||
|---|---|---|---|
PepSwift RP | |||
| Typical Applications | Peptides, proteins, and oligonucleotides | ||
| Column Dimensions | 100 μm × 50 mm | 200 μm × 50 mm | 500 μm × 50 mm |
| Base Material | Polystyrene/divinyl benzene | Polystyrene/divinyl benzene | Polystyrene/divinyl benzene |
| Chemistry | Phenyl | Phenyl | Phenyl |
| Recommended Flow Rate | 700–1000 nL/min | 2–3 μL/min | 15–25 μL/min |
| Temperature | 80 ?C | 80 ?C | 80 ?C |
| Maximum Pressure | 4350 psi (30 MPa) | 4350 psi (30 MPa) | 4350 psi (30 MPa) |
| Recommended Sample Quantity | 5 ng* | 20 ng* | 0.12 μg* |
| Sample Capacity*** | 0.2 ng | 0.6 ng | 3.8 ng |
| pH Stability | 1–10 | 1–10 | 1–10 |
| Solvent Compatibility | Most common RP solvents | Most common RP solvents | Most common RP solvents |
*Tryptic digest of six proteins
**E. coli proteins
*** Dynamic loading capacity is defined as the 10% increase in peak width measured at half height induced by injected mass of a single peptide (tryptic peptide of cytochrome c for PepSwift RP) and intact protein (cytochrome c for ProSwift RP-10R), respectively