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The Acclaim® PepMap100 C18 column sets the benchmark in peptide separation. The PepMap100 C18 column is available in 3 or 5 µm particle sizes, with 100 Å pore sizes, and in nano, capillary, and micro formats. nanoViper fingertight fittings allow for easy column installation.
Acclaim PepMap for TFA-Free Peptide Mapping Specifications | |||
---|---|---|---|
Column type | Nano | Capillary | Micro |
Column i.d. | 75 µm | 300 µm | 1.0 mm |
Length | 50, 150, 250, and 500 mm | 50, 150, and 250 mm | 50, 150, and 250 mm |
Recommended Flow Rate | 200 nL/min | 4 µL/min | 40 µL/min |
Packing Material | C18, 100 Å, 3 µm | C18, 100 Å, 3 µm | C18, 100 Å, 3 µm |
C18, 300 Å, 5 µm | C18, 300 Å, 5 µm | C18, 300 Å, 5 µm | |
C8, 100 Å, 3 µm | C8, 100 Å, 3 µm | C8, 100 Å, 3 µm | |
C8, 100 Å, 5 µm | C8, 100 Å, 5 µm | C8, 100 Å, 5 µm | |
C4, 300 Å, 5 µm | C4, 300 Å, 5 µm | C4, 300 Å, 5 µm | |
Column | Fused-silica | Glass Lined Tubing | Stainless steel |
Column Inlet | 1/16-in. PEEK sleeve adapter (provided) nanoViper (optional) | 1/16-in. universal fitting nanoViper (optional) 50µm i.d. PEEK sheathed fused silica with nanoViper fittings | 1/16-in. PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings |
Column Outlet | Fused-silica 25-µm i.d./ 280-µm o.d. | Fused-silica 75-µm i.d./ 280-µm o.d | 1/16-in. PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings |
MS Outlet | 1-m Length |