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Enzo Life Sciences公司的NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)和NUCLEAR-ID ® Red DNA Stain是細胞滲透性染料,適用于活細胞的細胞核染色。這系列染料為通過流式細胞術(shù)研究細胞周期進程的誘導(dǎo)和抑制提供了一種簡便方法。該試劑在活細胞研究中的潛在應(yīng)用包括確定細胞DNA含量和細胞周期分布、檢測生長模式的變化、監(jiān)測細胞凋亡以及評估腫瘤細胞行為和抑制基因機制。
NUCLEAR-ID® Red DNA stain
產(chǎn)品特點
● 遠紅色熒光特異性DNA染料
● 穩(wěn)定且純度高
● 可分析活細胞、可滲透細胞和固定細胞
● 無光漂白作用
● 無需RNase處理
● 與GFP和FITC兼容
● 不需要紫外線激光源激發(fā)
● 經(jīng)驗證適用于各種細胞密度
● 快速且易于使用
實例分析
在37°C下用終濃度為4000x或2000x的NUCLEAR-ID ® Red DNA Stain或同等µM濃度的競爭品牌染料對細胞進行染色,并在染色后輕輕洗滌。在15分鐘和24小時對細胞成像,結(jié)果顯示NUCLEAR-ID ® Red DNA Stain在更低的濃度就能顯現(xiàn)dsDNA。
產(chǎn)品詳情:
產(chǎn)品名稱 | NUCLEAR-ID® Red DNA stain |
產(chǎn)品貨號 | ENZ-52406 |
產(chǎn)品規(guī)格 | 200µl |
最大激發(fā)波長 | 566nm |
最大發(fā)射波長 | 650nm |
純度 | ≥93%(HPLC) |
質(zhì)量控制 | 1.Absorption peak of NUCLEAR-ID® Red dye: λmax = 566 ± 4 nm 2.% purity of NUCLEAR-ID® Red dye by HPLC: ≥93% |
應(yīng)用 | Flow Cytometry, Fluorescent detection |
短期儲存 | -20°C |
長期儲存 | -80°C |
產(chǎn)品說明 | The NUCLEAR-ID® Red DNA Stain is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
部分產(chǎn)品文獻引用
1. CD200R1L is a functional evolutionary conserved activating receptor in human neutrophils: M.I.P. Ramos, et al.; J. Leukoc. Biol. 111, 367 (2022)
2. FOXM1 network in association with TREM1 suppression regulates NET formation in diabetic foot ulcers: A.P. Sawaya, et al.; EMBO Rep. 23, e54558 (2022)
3. Multi-dimensional-double-spiral (MDDS) inertial microfluidic platform for sperm isolation directly from the raw semen sample: H. Jeon, et al.; Sci. Rep. 12, 4212 (2022)
4. DYRK1A negatively regulates CDK5-SOX2 pathway and self-renewal of glioblastoma stem cells: B. Chen, et al.; Int. J. Mol. Sci. 22, 4011 (2021)
5. Global phosphoproteomics reveals DYRK1A regulates CDK1 activity in glioblastoma cells: A. Recasens, et al.; Cell Death Discov. 7, 81 (2021)
6. Hydrogen Peroxide Is Crucial for NLRP3 Inflammasome-Mediated IL-1β Production and Cell Death in Pneumococcal Infections of Bronchial Epithelial Cells; Journal of Innate Immunity: S. Surabhi, et al.; J. Innate Immun. 10, 1159 (2021)
7. Licochalcone A inhibits hypoxia-inducible factor-1α accumulation by suppressing mitochondrial respiration in hypoxic cancer cells: M.K. Park, et al.; Biomed. Pharmacother. 133, 111082 (2021)
NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)
產(chǎn)品特點
● 藍色熒光特異性DNA染料
● 穩(wěn)定且純度高
● 可分析活細胞、可滲透細胞和固定細胞
● 無光漂白作用
● 無需RNase處理
● 與GFP和紅色染料兼容
● 經(jīng)驗證適用于各種細胞密度
● 快速易用
產(chǎn)品詳情:
產(chǎn)品名稱 | NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®) |
產(chǎn)品貨號 | ENZ-CHM103-0200 |
產(chǎn)品規(guī)格 | 200µl |
最大激發(fā)波長 | 350nm |
最大發(fā)射波長 | 461nm |
純度 | ≥95% (HPLC) |
外觀 | Frozen liquid |
應(yīng)用 | Flow Cytometry, Fluorescent detection |
短期儲存 | -20°C, Protect from light. Avoid freeze/thaw cycles |
長期儲存 | -80°C, Protect from light. Avoid freeze/thaw cycles |
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