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        1. 深圳欣博盛生物科技有限公司
          中級(jí)會(huì)員 | 第4年

          13129540117

          Epicypher欣博盛生物—CUTANA™ E. coli Spike-in DNA

          時(shí)間:2023/11/8閱讀:341
          分享:

          來源于Escherichia coli(E.coli)的片段DNA可以用作核酸酶靶向切割和釋放(CUT&RUN)的實(shí)驗(yàn)標(biāo)準(zhǔn)化的spike-in對(duì)照。產(chǎn)品CUTANA™ E. coli Spike-in DNA含有足夠的材料,可用于100-200個(gè)CUT&RUN樣本(高豐度和低豐度靶標(biāo)的范圍)。


          產(chǎn)品詳情

          保存溫度: Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -20°C.

          運(yùn)輸溫度: Room temp. Can go on frozen cold packs or dry ice.

          產(chǎn)品形式: 100 ng lyophilized DNA. *NOTE: May not be visible.


          驗(yàn)證數(shù)據(jù)

          Epicypher熱銷產(chǎn)品——CUTANA™ E. coli Spike-in DNA

          Figure 1: DNA gel data

          E. coli fragment size distribution. Lane 1: gDNA extracted from JM101 E. 

          coli cells (500 ng). Lane 2: Digested and purified CUTANA™ E. coli Spike-in 

          DNA (500 ng) resolved via 2% E-Gel™ EX Agarose Gel. Migration positions 

          of DNA molecular weight markers are indicated.


          Epicypher熱銷產(chǎn)品——CUTANA™ E. coli Spike-in DNA

          Figure 2: CUT&RUN sequencing data

          CUTANA™ E. coli Spike-in DNA (1.0 ng) was added to CUT&RUN samples using 500,000 K562 cells enriched 

          for a low abundance target (H3K4me3, EpiCypher 13-0041), a high abundance target (H3K27me3, EpiCypher 

          13-0030) and an IgG negative control (EpiCypher 13-0042). Total numbers of paired-end sequencing reads, reads 

          aligned to E. coli, and percentage of total sequencing reads aligned to E. coli spike-in DNA are shown. NOTE: 

          Spike-in DNA amount should be optimized by the end user with the goal of E. coli DNA comprising ~1% (0.2 - 

          5%) of total sequencing reads.


          訂購詳情

          貨號(hào)

          產(chǎn)品名稱

          規(guī)格

          18-1401

          CUTANA™ E. coli Spike-in DNA

          100 ng





          Epicypher熱銷產(chǎn)品——CUTANA™ pAG-Tn5 for CUT&Tag

          如需了解更多詳細(xì)信息或相關(guān)產(chǎn)品,請(qǐng)聯(lián)系EpiCypher中國授權(quán)代理商-欣博盛生物 

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