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Dionex AminoPac PA10氨基酸分析色譜柱
用途和特點
AminoPac PA10柱結(jié)合陰離子交換，梯度淋洗以及脈沖電化學檢測，可以分離多種氨基酸。分離氨基酸的同時，還可檢測氨基糖及碳水化合物。此外，還可分離多種糖類，磷酸化氨基酸，以及含硫氨基酸的普通氧化產(chǎn)物，如磺基丙氨酸。其柱填料為疏水性高聚物薄殼型陰離子交換劑，可在pH0-14范圍內(nèi)使用。填料*的pH穩(wěn)定性允許使用的淋洗液，有助于氨基酸在金電極的陽極氧化。
Dionex AminoPac PA10氨基酸分析色譜柱
訂貨信息：
| 分析柱 | |
| AminoPac PA10 Analytical Column (2 x 250 mm) | 055406 |
| AminoPac PA10 (22 x 250 mm) | SP5488 |
| AminoPac PA10 (9 x 250 mm) | SP5490 |
| AminoPac PA10 Analytical Column (4 x 250 mm) | SP5678 |
| 保護柱 | |
| AminoPac PA10 Guard Column (2 x 50 mm) | 055407 |
| AminoPac PA10 Guard Column (4 x 50 mm) | SP5680 |
| 配件 | |
| AAA-Direct Installation Kit | 059539 |
| Carbohydrate Removal Cartridge (CRC) for AAA-Direct | 068598 |
For High-Resolution Separations of Free Amino Acids by AAA-Direct
The AminoPac® PA10 column is packed with a hydrophobic, polymeric, pellicular, anion-exchange resin that is stable over the range of pH 0–14. The unique pH stability allows the use of eluents that are conducive to anodic oxidation of amino acids at gold electrodes. This column is recommended for use with the AAA-Direct™ Amino Acid Analyzer, allowing direct detection of primary and secondary amino acids by IPAD, with no need for pre- or postcolumn derivatization.
Derivatization not required
Carbohydrates and amino acids separated and detected simultaneously
Mid-femtomole to low-picomole detection limits
Compatible with all commonly used hydrolysis procedures
Can be configured for on-line analysis
AAA-Direct is a technique that eliminates the need for any form of derivatization, and is therefore compatible with on-line monitoring of cell cultures and fermentation broths.
The AminoPac PA10 column is optimized for separations of free amino acids by AAA-Direct. Vitamins, amino sugars, and carbohydrates can be detected simultaneously with amino acids. Additional capabilities include separation of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids, such as cysteic acid, methionine sulfone, and methionine sulfoxide.
| AminoPac PA10 Column for Amino Acid Analysis by AAA-Direct Specifications | |
|---|---|
| Recommended operating temperature | 30 °C |
| Typical operating conditions | 0.25 mL/min gradient Recommended flow rate: 0.25 mL/min |
| Mobile phase compatibility | pH 0–14; 100% compatible with common organic solvents. Dionex sodium acetate ionic form eluents only. |
| Resin composition | 8.5-μm-diameter microporous (<10 ?) bead (55% cross-linked) agglomerated with an 80-nm microbead alkyl ammonium functionalized latex (1% cross-linked). |
| Anion-exchange capacity | 60 μeq/column (2 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |
| Dimensions | AminoPac PA10 Analytical Column: 2 × 250 mm AminoPac PA10 Guard Column: 2 × 50 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
