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X-controller 發(fā)酵體系控制系統(tǒng)
Terrafors-IS Bacteria 固態(tài)樣品發(fā)酵罐
Techfors-S Bacteria 小型中試原位滅菌發(fā)酵系罐
Multifors Bacteria 平行發(fā)酵系統(tǒng)
Labfors 5 Bacteria 實驗室臺式旗艦型小型發(fā)酵罐
Minifors Bacteria 實驗室臺式標(biāo)準(zhǔn)發(fā)酵罐
Labfors Lux LED Flat Panel 平板LED 光照發(fā)酵罐
Labfors BioEtOH 臺式發(fā)酵罐(生物燃料專用)
MoFlo Astrios EQ 超高速流式分選系統(tǒng)
Tube, with Snap-On Cap, Polyethylene, 250 µL
Bio-Vial™, Tube, Polypropylene, 4 mL
Stack, Four-Shelf Side Loader試管架
Tube, Thickwall, Polycarbonate, 50 mL
Tube, with Snap-On Cap, Polycarbonate, 50 mL
Tube, Thinwall, Ultra-Clear™, 15.0 mL
Tube, Quick-Seal™, Polyallomer, 1.2 mL
g-Max Kit, Quick-Seal™, Polyallomer, 6.0 mL
g-Max Kit, Quick-Seal™, Polyallomer, 3.5 mL
g-Max Kit, Quick-Seal™, Polyallomer, 5.1 mL
Tube, OptiSeal™, Polyallomer, 4.7 mL
Tube, OptiSeal™, Polyallomer, 36.2 mL
Tube, Thickwall, Polyallomer, 3.2 mL
Tube, Quick-Seal™, Polyallomer, 5.1 mL
Tube, OptiSeal™, Polyallomer, 4.9 mL
Tube, Thinwall, Polyallomer, 15.0 mL
Tube, OptiSeal™, Polyallomer, 3.3 mL
Tube, OptiSeal™, Polyallomer, 8.9 mL
Tube, OptiSeal™, Polyallomer, 32.4 mL
Tube, OptiSeal™, Polyallomer, 11.2 mL
g-Max Kit, Quick-Seal™, Polyallomer, 27 mL
Tube, Quick-Seal™konical™Polyallomer8mL
Tube, Quick-Seal™,konical™, Polyallomer, 28 mL
Tube, Quick-Seal™,konical™, Polyallomer, 1.5 mL
Tube, Quick-Seal™, Polyallomer, 3.9 mL
Tube, Quick-Seal™,konical™, Polyallomer, 8.5 mL
Tube, Quick-Seal™,konical™, Polyallomer, 4 mL
Tube, Quick-Seal™,konical™, Polyallomer, 1.3 mL
Tube, Quick-Seal™,konical™, Polyallomer, 23 mL
Tube, Quick-Seal™,konical™, Polyallomer, 3 mL
Tube, Thinwall,konical™, Polyallomer, 1.5 mL
Tube, Thinwall,konical™, Polyallomer, 10 mL
Tube, Thinwall,konical™, Polyallomer, 14.5 mL
Tube, Thinwall,konical™, Polyallomer, 25 mL
Tube, Thinwall,konical™, Polyallomer, 30 mL
Tube, Quick-Seal™,konical™, Polyallomer, 3.2 mL
Tube, Thinwall,konical™, Polyallomer, 3 mL
Tube, with Snap-On Cap, Polypropylene, 50 mL
Tube, with Snap-On Cap, Polypropylene, 1.5 mL
g-Max Kit, Quick-Seal™, Polyallomer, 4.2 mL
g-Max Kit, Quick-Seal™, Polyallomer, 2.0 mL
g-Max Kit, Quick-Seal™, Polyallomer, 15 mL
Bottle, Polycarbonate, 10.4 mL
Tube, Thickwall, Polyallomer, 81 mL
Tube, Thickwall, Polyallomer, 32 mL
Tube, Thickwall, Polycarbonate, 4 mL
Tube, Polyallomer, Thickwall, 3.5 mL
Tube, Quick-Seal™, Polyallomer, 4.2 mL
Tube, Thickwall, Polyallomer, 6.5 mL
Tube, Quick-Seal™, Polyallomer, 17 mL
Tube, Thickwall, Polycarbonate, 6.5 mL
Tube, Thickwall, Polycarbonate, 1.0 mL
Tube, Thickwall, Polyallomer, 4 mL
Bottle Assembly, Polycarbonate, 500 mL
Tube, Quick-Seal™, Polyallomer, 3.5 mL
Tube, Thickwall, Polyallomer, 10 mL
Tube, Thickwall, Polycarbonate, 32 mL
Bottle, Assembly, Polycarbonate, 70 mL
Tube, Thickwall, Polycarbonate, 10 mL
Tube, Thickwall, Polycarbonate, 3 mL
Tube, Thickwall, Polyallomer, 3 mL
Tube, Thickwall, Polyallomer, 10.5 mL
Tube, Thickwall, Polycarbonate, 81 mL
Tube, Thickwall, Polycarbonate, 3.5 mL
Bottle, with Cap Assembly, Polycarbonate, 10.4 mL
Bottle Assembly, Polycarbonate, 70 mL
Tube, Thickwall, Polyallomer, 3.5 mL
Tube, Thickwall, Polycarbonate, 10.5 mL
Bottle, with Cap Assembly, Polycarbonate, 26.3 mL
Tube, Quick-Seal™, Polyallomer, 5.9 mL
Bottle Assembly, Polycarbonate, 26.3 mL
Tube, Thinwall, Polyallomer, 2.2 mL
Tube, Thinwall, Ultra-Clear™, 800 µL
Tube, Quick-Seal™, Ultra-Clear™, 6.0 mL
Tube, Thickwall, Polyallomer, 500 µL
Tube, Quick-Seal™, Ultra-Clear™, 27 mL
Tube, Quick-Seal™, Polyallomer, 8 mL
Tube, Quick-Seal™, Polyallomer, 10 mL
Ultra Low Endotoxin Fetal Bovien Serum超低內(nèi)毒素胎牛血清
Ultra Low Endotoxin Fetal Bovien Serum
超低內(nèi)毒素胎牛血清
內(nèi)毒素(Endotoxin)是革蘭氏陰性細菌細胞壁中的一種成分,叫做脂多糖體(Lipopolysaccharide, LPS).內(nèi)毒素只有當(dāng)細菌死亡溶解或用人工方法破壞菌細胞后才釋放出來,所以叫做內(nèi)毒素.內(nèi)毒素是所有細胞培養(yǎng)的大敵,對細胞培養(yǎng)的影響性早在將近三十年前就被廣泛討論和重視。
一般來說,細胞或組織培養(yǎng)過程中內(nèi)毒素污染源通常來自于血清、培養(yǎng)液及外加藥物配制用水等。內(nèi)毒素對細胞的影響根據(jù)細胞株種類的不同會有所差異,其影響性主要包括一下幾方面:
1. 改變細胞外形,如造成肝細胞外形改變并引發(fā)其吸收六碳糖的能力降低。
2. 活化細胞,產(chǎn)生訊息傳遞物質(zhì),并影響其下游生物途徑反應(yīng).如內(nèi)毒素會活化巨噬細胞使其分泌腫瘤壞死因子(Tumor Necrosis Factor,TNF)、干擾素(Interferon,IFN),及白細胞介素1(IL-1)等細胞因子,這些細胞因子作用于宿主下丘腦的體溫調(diào)節(jié)中樞,促使體溫升高發(fā)熱。
3. 抑制酵素活性,如血管收縮素轉(zhuǎn)化酶(Angiotensin Converting Enzyme)。
4. 促進或抑制細胞分裂,其效果差異可能與內(nèi)毒素來源于濃度有關(guān)。
5. 抑制人工受精 (In Vitro Fertilization) 的卵子受精率。
6. 其他方面:如影響細胞附著力、降低肝細胞對胰島素(Insulin)結(jié)合力、降低細胞鉀離子流失等。
由于內(nèi)毒素可以帶來眾多難以預(yù)期的不良后果,因此,研究者若想得到準(zhǔn)確的實驗結(jié)果,應(yīng)謹慎操作,避免細胞或組織培養(yǎng)系統(tǒng)受到內(nèi)毒素污染的影響,如可選擇內(nèi)毒素更低的蒸餾水用于細胞培養(yǎng)相關(guān)試劑的配制,選擇優(yōu)質(zhì)的培養(yǎng)基及來源可靠的血清等,從而在源頭上將內(nèi)毒素污染幾率降到低,保障實驗順利進行。
上一般要求胎牛血清的內(nèi)毒素含量不超過10EU/ml,市面上品質(zhì)好一點的胎牛血清內(nèi)毒素含量在1EU/ml;而超低內(nèi)毒素含量的則要求要小于0.5EU/ml,目前人們常把內(nèi)毒素低于0.5EU/ml含量的胎牛血清稱為超低內(nèi)毒素胎牛血清。
NANOLAB公司推出的超低內(nèi)毒素含量一般在0.2~0.3EU/ml,*符合細胞培養(yǎng)過程中的各種要求,比如,防止血清中過多的內(nèi)毒素導(dǎo)致細胞被激活、活化,細胞過早凋亡,衰老速度過快等。
產(chǎn)品信息:
品牌 | 貨號 | 產(chǎn)品名稱 | 規(guī)格 | 單位 | 市場價格 |
NANOLAB | 01-06-1A | Ultra Low Endotoxin Fetal Bovien Serum 超低內(nèi)毒素胎牛血清 | 500ml/瓶 | 瓶 | 6500 |
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