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Source

Rabbit

Applications

WB,ELISA

Reactivity

Human,Mouse

Dilution

WB: 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

Storage

-20°C/1 year

Specificity

Phospho-CD28 (Y218) Polyclonal Antibody detects endogenous levels of CD28 protein only when phosphorylated at Y218.

Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen

The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218. AA range:171-220

Uniprot No

P10747

Alternative names

CD28; T-cell-specific surface glycoprotein CD28; TP44; CD antigen CD28

Form

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Background

CD28 molecule(CD28) Homo sapiens The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011],

Other

CD28, T-cell-specific surface glycoprotein CD28

Mol.Wt (Da)

25066

Concentration

1 mg/ml