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        1. 靶點科技(北京)有限公司

          當(dāng)前位置:> 供求商機> 抗嘌呤霉素抗體Anti-Puromycin [3RH11] Antibody

          [供應(yīng)]抗嘌呤霉素抗體Anti-Puromycin [3RH11] Antibody

          更新時間:2024-11-19 21:00:07

          有效期:2024年11月19日 -- 2025年5月19日

          已獲點擊:32

          聯(lián)系我時,請告知來自 化工儀器網(wǎng)

          Thismonoclonalantibodytopuromycinprovidesanon-radioactivemethodtomeasureratesofglobalproteinsynthesis(mRNAtranslation)incellsortissueslicesincubatedwithpuromycin

          This monoclonal antibody to puromycin provides a non-radioactive method to measure rates of global protein synthesis (mRNA translation) in cells or tissue slices incubated with puromycin, or animals treated with puromycin in vivo.

          Highlights:

          - Allows for the simple evaluation and quantification of translation directly using standard immunochemical methods

          - Advantageous alternative to traditional pulse-chase methods, which rely on radioactive amino acid labeling

          - Compatible with Western Blot and ELISA applications

          Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome. Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Classical pulse-chase or flooding dose methods used to monitor protein synthesis rely on the measurement of radioactive methionine and cysteine labels. Analysis using puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling, and allows for the evaluation/quantification of translation directly using standard immunochemical methods.

          Product Type:Antibody
          Name:Anti-Puromycin (3RH11)
          Host:Mouse
          Isotype:IgG1-k
          Clonality:Monoclonal
          Clone Name:3RH11
          Specificity:This antibody recognizes puromycin.
          Immunogen:puromycin hydrochloride
          Format:Liquid
          Purification Method:Protein G purified
          Buffer:PBS
          Tested Applications:Western blotting (1:1,000), ELISA and Immunofluorescence microscopy.
          Storage:+4C (short-term), -20C (long-term); Avoid repeated freeze/thaw cycles.
          Shipped:Cold packs


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