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PC-12細(xì)胞

細(xì)胞名稱： 大鼠腎上腺嗜鉻細(xì)胞瘤細(xì)胞(未分化)

細(xì)胞別名：PC-12; PC 12

貨號：TCR-C617

規(guī)格： 1X10^6

價格：1500 元（推薦T25瓶，復(fù)蘇發(fā)貨1-2周）

PC-12細(xì)胞細(xì)胞系來自可移植的雄性大鼠的腎上腺嗜鉻細(xì)胞瘤；表達(dá)神經(jīng)生長因子(NGF)受體，NGF可誘導(dǎo)該細(xì)胞產(chǎn)生可逆性的神經(jīng)表型。該細(xì)胞可產(chǎn)生兒茶酚胺、多巴胺和去甲腎上腺素，但不合成腎上腺素。

種屬來源：大鼠

組織來源：腎上腺

疾病特征：嗜鉻細(xì)胞瘤

細(xì)胞形態(tài)：圓形

生長特性：疏松貼壁和懸浮同時存在

PC-12細(xì)胞培養(yǎng)體系：RPMI-1640+5%FBS+15%馬血清+1%P/S

配套培養(yǎng)基貨號：TCR-G617

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲存

質(zhì)量檢測：細(xì)菌、真菌、支原體檢測均為陰性

參考文獻(xiàn)：

Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317

Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897

Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712

Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125

U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005

Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897