詳細(xì)介紹
三糖鐵瓊脂培養(yǎng)基(USP)
英文名稱: Triple SugarIronAgar Medium
產(chǎn)品貨號: HB4088-2
產(chǎn)品規(guī)格: 250g
產(chǎn)品價格: 130元
產(chǎn)品用途: 用于腸桿菌科細(xì)菌的生化反應(yīng)篩選
成分(g/L):
Pancreatic Digest of Casein/胰酪蛋白胨 | 10.0 |
Peptic Digest of Animal Tissue/胃酶消化動物組織 | 10.0 |
Sodium Chloride /氯化鈉 | 5.0 |
Lactose/乳糖 | 10.0 |
Dextrose/葡萄糖 | 1.0 |
Sucrose/蔗糖 | 10.0 |
Phenol Red/酚紅 | 0.025 |
Ferrous Ammonium Sulfate/硫酸亞鐵銨 | 0.2 |
Sodium thiosulfate/硫代硫酸鈉 | 0.2 |
Agar/瓊脂 | 13.0 |
pH值7.3 ± 0.2 | 25℃ |
檢驗(yàn)原理
胰酪蛋白胨、胃酶消化動物組織提供氮源、維生素、礦物質(zhì);乳糖、葡萄糖、蔗糖為可發(fā)酵糖類,其產(chǎn)酸時通過酚紅指示劑測出,酸性呈黃色,堿性呈紅色;硫代硫酸鈉可被某些細(xì)菌還原為硫化氫,與硫酸亞鐵中的鐵鹽生成黑色硫化鐵;氯化鈉維持均衡的滲透壓;瓊脂是培養(yǎng)基的凝固劑。
用法:
Suspend 64.5g/liter, heat to boilling for 1 min with shaking. Fill into tubes to one-third of their height, sterilise by heating in an autoclave at 121℃ for 15 min and allow to cool in a position that gives a deep portion and a sloping surface.
稱取本品 64.5g,加熱溶解于1000ml 純化水中,加熱煮沸 1分鐘,分裝于 16mm× 150mm試管,121℃高壓滅菌 15 分鐘制成高層斜面。