The PARP15 Chemiluminescent Assay Kit is designed to measure PARP15 activity for screening and profiling applications. The key to the PARP15 Chemiluminescent Assay Kit is the biotinylated substrate. With this kit, only three simple steps are required for PARP15 reactions. First, histone proteins are coated onto the wells of the strip-plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP15 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.