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當(dāng)前位置:BPS Bioscience Inc.>>Coronavirus Assay Kits>> 78847Fluoro-Verse™ ACE2 Inhibitor Assay Kit
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產(chǎn)品型號78847
品 牌BPS Bioscience
廠商性質(zhì)生產(chǎn)商
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更新時(shí)間:2023-11-12 17:24:17瀏覽次數(shù):113次
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The Fluoro-Verse™ Angiotensin Converting Enzyme 2 (ACE2) Inhibitor Assay Kit is a 96-well homogeneous fluorogenic assay designed to measure the exopeptidase activity of ACE2 for screening and profiling applications. The ACE2 assay kit contains enough purified ACE2 (BPS Bioscience #11003, amino acids 18-740), its substrate, and ACE2 buffer for 100 reactions.
Figure 1: Illustration of the principle behind the ACE2 fluorogenic assay.
The assay uses an ACE2 substrate in which the fluorescence emitted by Mca (7-Methoxycoumarin-4-acetic acid) is quenched by the proximal Dnp (2,4-dinitrophenyl) quencher. ACE2 is a carboxypeptidase that cleaves the C-terminus peptide bond. Thus, Mca is released from quenching upon proteolysis and emits fluorescence with excitation/emission maxima of 320/380 nm. The increase in fluorescence is proportional to ACE2 activity.
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