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        1. 北京云肽生物科技有限公司
          中級(jí)會(huì)員 | 第1年

          13269192326

          生化試劑
          伊勢(shì)久 源葉 Santa 博邁德 亞科因 ?Proteintech/武漢三鷹 陶術(shù) 索萊寶 普利萊 美天旎 麥克林 阿拉丁 OXOID Elabscience DSHB 伯樂(lè) Peprotech Progen Polyplus polysciences Rockland Streck Sigma-Aldrich Serumwerk Bernburg AG Stressmarq SurModics Sangon Biotech SBA TBD TAKARA The Well Vector Laboratories U-CyTech WAKO Yakult 安度斯 碧云天 梅里埃API AnaSpec Abcam Absolute Antibody ADI Bethyl Biowest Borer Bovogen CLARK CellArtis Cayman Cedarlane Diagenode EliteCell Epigentek Fitzgerald Gemini Gibco Hyclone Hospira StemCell Hamilton Helios IBA Irvine ICT Keygen Logos Lucigen AppliChem Atlas antibodies Avidity LONZA龍沙 Luminex Mettler/梅特勒托利多 Mirus MoBio MP Biomedicals Mesalabs MabTech Nordmark Nordic-MUbio Nanoprobes NanoTag nanoComposix 源培 櫻花 義翹神州 OMEGA promega Zymo Research 四季青 中國(guó)臺(tái)灣旭基 天根 同仁化學(xué) 中杉金橋
          儀器耗材

          ARBOR ASSAYS 免疫測(cè)試產(chǎn)品 試劑盒-相關(guān)技術(shù)文章

          時(shí)間:2024/7/13閱讀:189
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          ARBOR ASSAYS 免疫測(cè)試產(chǎn)品 試劑盒


          Arbor Assays公司成立于2007年,位于密歇根州的安娜堡,公司的三位主創(chuàng)始人均為有多年科研經(jīng)驗(yàn)的生命和臨床科學(xué)家。其宗旨是為臨床使用的重要生物分子建立高質(zhì)量的檢測(cè)和免疫測(cè)試產(chǎn)品。在生物領(lǐng)域建立健全的免疫檢測(cè)和酶活性試劑盒以定量生物分子。

          主要為臨床重要生物分子提供高質(zhì)量的檢測(cè)和免疫測(cè)試產(chǎn)品,如乙酰膽堿酯酶活性熒光檢測(cè)試劑盒、谷胱甘肽熒光檢測(cè)試劑盒、血紅蛋白比色檢測(cè)試劑盒等。種類多樣,操作簡(jiǎn)單,靈敏度高。

          認(rèn)識(shí)到對(duì)靈敏度、速度、便利性、質(zhì)量和服務(wù)的需求,我們研究、開(kāi)發(fā)和制造用于臨床重要生物分子的最高質(zhì)量的檢測(cè)和免疫測(cè)定試劑盒。

          Arbor Assays公司的主要產(chǎn)品包括:

          DetectX® Kits

          Inhibitors & Activators

          AbX® Antibodies & Affinity Resins

          Detection Systems

          Components & Ancillary Reagents

          所需材料:

          類固醇標(biāo)準(zhǔn)品,用于檢測(cè)萃取效率

          ACS級(jí)乙醇(或乙酸乙酯)

          玻璃試管

          處理流程:

          確保樣品wan全干燥,并對(duì)樣品進(jìn)行粉末化處理,以提高萃取回收率。如果可能的話清除任何大顆粒,如草屑。我們建議通過(guò)使用試劑盒的AB分析緩沖液及已知濃度的類固醇溶液檢測(cè)結(jié)果來(lái)確定提取效率,詳細(xì)信息請(qǐng)參考下面的萃取效率部分。乙醇或乙酸乙酯萃取樣品及萃取效率控制如下:

          1.稱取≥0.2 g干燥的糞便固體放入管中。樣品可以通過(guò)被動(dòng)干燥、溫和加熱(60°C)或冷凍的方式來(lái)進(jìn)行干燥處理。

          2.0.1 g固體(0.1 g糞便固體/mL)加入1 mL乙醇(或乙酸乙酯)并密封。

          3.用力搖晃至少30分鐘。

          4.4°C下,將樣品在5,000 rpm下離心15分鐘。取上清放入一個(gè)干凈的管中。如果密封得當(dāng),該樣本可在≤-20°C下保存至少一個(gè)月。

          注:含有低水平分析物的樣品可以通過(guò)干燥提取液和在少量分析緩沖液中重懸,來(lái)達(dá)到濃縮的效果。用乙酸乙酯提取的樣品在加入緩沖液之前,必須wan全干燥以去除溶劑。從步驟4中轉(zhuǎn)移測(cè)量體積(蒸發(fā)體積)的上清到一個(gè)干凈的管中,在離心蒸發(fā)裝置或氮?dú)庀抡舭l(fā)至干燥。干燥、提取的樣品可在干燥器中冷凍≤-20°C保存?;蛴靡掖?span>(100μL)溶解干燥提取的樣品,直接進(jìn)行步驟5。

           5.以上步驟為通用步驟,可根據(jù)需要量進(jìn)行等比例體積調(diào)整。將步驟4中提取的樣本上清液100μL加入最少400μLAB緩沖液中(總重構(gòu)體積=100μL+400μL)。室溫下渦旋混勻,靜置5min,重復(fù)至少兩次渦旋和靜置步驟。對(duì)于免疫分析,測(cè)定中乙醇含量通常需要≤5%,需要根據(jù)所選擇的試劑盒中描述的最大乙醇含量耐受情況,用檢測(cè)緩沖液進(jìn)行稀釋。

          6.根據(jù)試劑盒說(shuō)明書對(duì)稀釋后的樣本進(jìn)行ELISA檢測(cè)。

           

          下面是ARBOR ASSAYS公司的部分熱銷產(chǎn)品

          產(chǎn)品名稱

          貨號(hào)

          規(guī)格

          Goat Anti-Mouse IgG Fc, 25MG

          A008-25MG

          25MG

          Cyclic AMP Direct ELISA Kit (1 Plate)

          K019-H1

          1x96 well plate

          Corticosterone ELISA Kit (1 Plate)

          K014-H1

          1x96 well plate

          Protein Kinase A (PKA) Activity Assay Kit (1 Plate)

          K027-H1

          1x96 well plate

          Aldosterone ELISA Kit (1 Plate)

          K052-H1

          1x96 well plate

          Urea Nitrogen (BUN) Detection Kit (2 Plate)

          K024-H1

          2x96 well plate

          Cortisol ELISA Kit (1 Strip plate)

          K003-H1

          1x96 well plate

          PGFM ELISA Kit (1 Plate)

          K022-H1

          1x96 well plate

          Triiodothyronine (T3) ELISA Kit (1 Plate)

          K056-H1

          1 Kit

          FRAP™ (Ferric Reducing Antioxidant Power) Detection   Kit (2 Plate)

          K043-H1

          2x96 well plate

          Hemoglobin Detection Reagent, 20ML

          C038-20ML

          20ML

          Ceruloplasmin Colorimetric Activity Kit (2 Plate)

          K035-H1

          2x96 well plate

          ThioStar™ Fluorescent Thiol Substrate, 100UG

          L002-100UG

          100UG

          Cyclic GMP Direct Chemiluminescent ELISA Kit (1   Plate)

          K020-C1

          1x96 well plate

          Progesterone ELISA Kit (1 Plate)

          K025-H1

          1x96 well plate

          Testosterone ELISA Kit (1 Plate)

          K032-H1

          1x96 well plate

          Estrone-3-Glucuronide (E1G) ELISA Kit (1 Plate)

          K036-H1

          1x96 well plate

          Thyroxine (T4) ELISA Kit (1 Plate)

          K050-H1

          1x96 well plate

          Prostaglandin E2 (PGE2) Multi-Format ELISA Kit (1   Plate)

          K051-H1

          1x96 well plate

          Tranylcypromine

          X042-1EA

          1EA

          Triiodothyronine (T3) ELISA Kit (5 Plate)

          K056-H5

          5x96 well plate

          Glutathione Colorimetric Detection Kit (4 Plate)

          K006-H1

          4x96 well plate

          Cyclic GMP Direct ELISA Kit (1 Plate)

          K020-H1

          1x96 well plate

          Progesterone ELISA Kit (5 Plate)

          K025-H5

          5x96 well plate

          Cyclic AMP Direct ELISA Kit (5 Plate)

          K019-H5

          5x96 well plate

          Estradiol ELISA Kit (1 Plate)

          K030-H1

          1x96 well plate

          Oxytocin ELISA Kit (1 Plate)

          K048-H1

          1x96 well plate

          Estrone-3-Glucuronide (E1G) ELISA Kit (5 Plate)

          K036-H5

          5x96 well plate

          Pregnanediol-3-Glucuronide (PDG) ELISA Kit (5   Plate)

          K037-H5

          5x96 well plate

          Hemoglobin Standard, 300UL

          C037-300UL

          300UL

          DetectX® Estrone-3-Sulfate (E1S) Antibody, 3ML

          C133-3ML

          3ML

          Creatinine Urinary Detection Kit (2 Plate)

          K002-H1

          2x96 well plate

          Butyrylcholinesterase Fluorescent Activity Kit (2   Plate)

          K016-F1

          2x96 well plate

          Corticosterone ELISA Kit, (5 Plate)

          K014-H5

          5x96 well plate

          Cyclic GMP Direct Chemiluminescent ELISA Kit (5   Plate)

          K020-C5

          5x96 well plate

          Superoxide Dismutase (SOD) Colorimetric Activity   Kit (2 Plate)

          K028-H1

          2x96 well plate

          Catalase Colorimetric Activity Kit (2 Plate)

          K033-H1

          2x96 well plate

          Glutathione Fluorescent Detection Kit (1 Plate)

          K006-F1

          1x96 well plate

          Pregnanediol-3-Glucuronide (PDG) Standard, 625UL

          C128-625UL

          625UL

          Thyroxine (T4) ELISA Kit (5 Plate)

          K050-H5

          5x96 well plate

          Acetylcholinesterase Fluorescent Activity Kit (2   Plate)

          K015-F1

          2x96 well plate

          Creatinine Serum Kit (2 Plate)

          KB02-H1

          2x96 well plate

          Hydrogen Peroxide Fluorescent Detection Kit (2   Plate)

          K034-F1

          2x96 well plate

          Creatinine Serum Low Sample Volume Kit (384-well   Plate)

          KB02-H1D

          1x384 well plate

          Recombinant Obelin, 100UG

          L001-100UG

          100UG

          Dry DMSO, 20ML

          X022-20ML

          20ML

          Dry DMSO, 2ML

          X022-2ML

          2ML

          Urea Nitrogen Standard, 1ML

          C089-1ML

          1ML

          NADPH lyophilized, 1EA

          X045-1EA

          1EA

          DetectX® PGE2 Conjugate, 13ML

          C179-13ML

          13ML

          17-Hydroxyprogesterone   ELISA Kits

          K053-H

          1 Kit

          Arg8-Vasopressin (AVP) ELISA Kits

          K049-H

          1 Kit

          Arg8-Vasopressin (AVP) Chemiluminescent   ELISA Kits

          K049-C

          1 Kit

          Androstenedione ELISA Kits

          K070-H

          1 Kit

          Aldosterone Chemiluminescent ELISA Kits

          K052-C

          1 Kit

          Allopregnanolone ELISA Kits

          K061-H

          1 Kit

          Adrenocorticotropic Hormone (ACTH) ELISA   Kits

          K072-H

          1 Kit

          Alkaline Phosphatase (ALP) Colorimetric   Activity Kit

          K082-H1

          1 Kit

          3,3-Cyclic GAMP ELISA Kits

          K073-H

          1 Kit

          20-Hydroxyecdysone ELISA Kit

          K066-H

          1 Kit

          2,3-Cyclic GAMP STING-Based FRET Detection Kits

          K081-F

          1 Kit

          2,3-Cyclic GAMP ELISA Kits

          K067-H

          1 Kit

          Cyclic GMP Direct ELISA Kits – Improved   Sensitivity

          K065-H

          1 Kit

          Endothelin-1 (ET-1) ELISA Kit

          K045-H1

          1 Kit

          Estrone-3-Sulfate (E1S) ELISA Kits

          K038-H

          1 Kit

          Assay Buffer, 5X

          X065-110ml

          110ml

          Blocking Buffer, 10X

          X109-25ml

          25ml

          Goat anti-Rabbit IgG Coated Plate

          X016-1EA


          HRP Conjugate Diluent

          X076-300ml

          300ml

          TMB Substrate

          X019-55ml

          55ml

          Mesotocin Solution (500ng/mL)

          X127-625UL


          5-Azacytidine

          P012-50MG

          50mg

           

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