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        1. 上海乾思生物科技有限公司
          中級會員 | 第16年

          18916423352

          細(xì)胞庫
          優(yōu)勢品牌
          Encapsula NanoSciences Quidel Bioporto Salimetrics Research Products International Corp Quanta BioDesign Peninsula Laboratories International Golden West Diagnostics Ocean NanoTech HaemoScan affinitybiologicals Cytodiagnostics Innovagen AB CLIAwaived Svar Abbexa ABMgood AffinityImmuno AGScientific AHF analysentechnik AG Alomone 英國Antibodies AstaTech Avidity Badrilla BioAcademia Biomatik Biosynth BioTeZ Biotrend Calbiotech CaroteNature Cell Sciences Click Chemistry Tools Cohesion Biosciences CordenPharma Cosmo Bio Creative Biomart Crystal Chem Crystalgen Diagnovum DiaMetra Dianova Diatheva Eagle Manufacturing Echelon Biosciences Edgebio Emfret Epoch Life Science Equitech-Bio Eramask Euro Diagnostica Exocell Fine Science Tools GenwayBio Giotto Biotech Glen Research Glycosynth Gold Biotechnology Golden West Biologicals Haemtech(HTI) Herolab IHC WORLD ImmuChrom GmbH Immunostep Immunotools Immunovision INCELL Corporation Innovative Research Innovrsrch(IRA) Insitus Iris Biotech GmbH Isosep IST Engineering Inc J.G. Finneran Kainos Kamiya Biomedical Labor Diagnostika Nord Larodan Laysanbio Lc laboratories Lee BioSolutions LGC Biosearch Technologies Lifecore List Labs lumiprobe Macrocyclics Medix Biochemica Meridian Life Science Microbix Milenia Biotec Moscerdam Mybiosource Nanoimmunotech Nanoprobes Neuro Probe Paratechs Physitemp Polypure Polysciences Probetex Progen ProScience Inc ProSpec QAbio Qorpak Quanta BioSciences QuickZyme RevMAb Biosciences SCHMIDT BioMedTech Seven Hills Foundation Smartox Biotechnology Spherotech Surgimax Swant SYSY Ted Pella Torpac Trilink USBio VIDIA Ltd ViroStat Virusys VP-scientific Xcessbio XENOMETRIX Zeptometrix ZYTOVISION
          自產(chǎn)ELISA試劑盒,試劑盒
          進口ELISA試劑盒
          人細(xì)胞,人細(xì)胞系,人細(xì)胞株
          3h biomedical,3H Biomedical AB,3hbiomedical,3-M Company,4adi,4rtilab,5 prime,5prime a & g pharmaceuticals,A&D Weighing,Aalto Scientific,Aalto Scientific ltd,Aalto Scientific,ltd.,aaltobioreagents,aaltoscientific,aat bioquest AAT Bioquest Inc,AB Biolabs,ab peptides,AB Sciex,Abaxis,Abazyme,Abberior,abbexa abbiotec,Abbkine,abbomax,Abbott Molecular,abcam,ABclonal,ABclonal Inc.,abcore-inc abcr GmbH,abcrGmbH,abcysonline,ABD,ABD Bioquest,Inc,abd serotec,ab-direct,abdserotec Abeomics,abfrontier,abgab,abgene,abgent,ABI Applied Biosystems,abiocode,abionline Abkem Iberia,ABL,Inc,Abliance,abm Inc.,abmart,abmgood,Abmole,Abmole Bioscience Inc. abnova,Abraxis,Absolute Antibody,ABT Beads,abt beads technologies,ABTbeads,abvision,Abways abzoom,academy biomed,academy bio-medical,acanthus research,acanthusresearch,ACC,accelagen,Accelagen inc. Access Biologicals,LLC,accessbiologicals,acciusa,accu-chek,AccuDiag Allergy,AccuDiag Anemia,AccuDiag Autoimmune Diseases,AccuDiag Bone Metabolism AccuDiag Thyroid,accumax accutase,Accumetrics,Accurate,accurate chemical,Accurate Wirecraft Company,accuratechemical,accustandard acdbio,Ace Glass,Ace Hose and Rubber Co,ACEA Biosciences,ACL Incorporated,ACME Microspheres Inc,ACMEmicrospheres,acris acris antibodies,Acris Antibodies GmbH,acris-antibodies,ACROBiosystems,acrometrix,acros,Acros Organics,ACT Test Panels LLC actc,actgene,active motif,activemotif,Adam Equipment,Inc,AddexBio,Addexbio Technologies,addgene ademtech,adg,ADI,ADInstruments,adipogen,Adolf Kuhner,adooq,Advance Tabco advanced immunochemical,advanced immunochemical inc,advanced targeting systems,Advansta,Advansta Inc.,Advansta,Inc,Advantech,Advent Electric Inc adventbio,advnt,advtechind,advy chemical,advy chemical Purified Proteins,advychemical Polyclonal Antisera,aesku,aetltd affiland,affinity,affinity biologicals,affinity bioreagent,Affinity BioReagents,Affinity BioReagents abr,Affinity Biosciences,affinitybiologicals AffinityBioReagents,AffinityImmuno,affix scientific,affixscientific,affymetrix,ag scientific,Agar Scientific,agarscientific agdia,Agela Technologies,agilent,Agilent Seahorse,agilent technologies,agilet,agilet solutions,agiletsolutions agrenvec,agrisera,AGRO-BIO,agscientific,ahdiagnostics,ahf,AHF analysentechnik,AHF analysentechnik AG Air Technology Solutions,Airgas Safety,Air-Tite,AJAX SCIENTIFIC LTD,AK Limited,Akro-Mils,ALA Scientfic,alamarblue Alba Bioscience,Alconox Inc,aldevron,alerchek,alere,Alexis,ALEXIS Biochemicals,Alfa Aesar Aline Biosciences,AlineBiosciences,All Foils Incorporated,Allcells,allele biotech,allelebiotech,allen-bradley,AllerMAbs
          大鼠細(xì)胞,大鼠細(xì)胞系
          細(xì)胞株,細(xì)胞系,傳代細(xì)胞
          白鰱細(xì)胞 斑馬魚細(xì)胞 豹貓細(xì)胞 成人細(xì)胞 赤狐細(xì)胞 大額牛細(xì)胞 大耳山羊細(xì)胞 大黃魚細(xì)胞 大長舌果蝠細(xì)胞 俄勒岡地鼠細(xì)胞 非洲綠猴細(xì)胞 高背鯽魚細(xì)胞 果蠅細(xì)胞 果子貍細(xì)胞 荷蘭黑白花奶牛細(xì)胞 黑化大家鼠細(xì)胞 花鼠細(xì)胞 家兔細(xì)胞 昆蟲細(xì)胞 綠猴細(xì)胞 馬鐵菊頭蝠細(xì)胞 綿羊細(xì)胞 三葉小蹄蝠細(xì)胞 散鱗鏡鯉細(xì)胞 社鼠細(xì)胞 鼠細(xì)胞 樹鼩細(xì)胞 團頭魴細(xì)胞 新生兒細(xì)胞 新生牛細(xì)胞 敘利亞倉鼠細(xì)胞 長尾綠猴細(xì)胞 中國倉鼠細(xì)胞 中華鱉細(xì)胞 棕果蝠細(xì)胞 腫瘤細(xì)胞 豬細(xì)胞 兔細(xì)胞 犬細(xì)胞
          抗體庫,小包裝,流式抗體
          SIGMA現(xiàn)貨,SIGMA代理

          細(xì)胞因子檢測技術(shù)

          時間:2011/10/13閱讀:990
          分享:

          乾思 生物編輯整理: 教 師:XXX 實驗類型:基礎(chǔ) 學(xué)  時:8 (參考) 內(nèi)  容: 一、實驗?zāi)康模?1.以檢測IL-2為例,使學(xué)生掌握一定的細(xì)胞培養(yǎng)技術(shù)。 2.使學(xué)生掌握一定的細(xì)胞因子檢測的一些主要方法。 3.掌握免疫細(xì)胞分離的主要技術(shù)和方法。 二、實驗原理: 檢測細(xì)胞因子對于闡明機體免疫應(yīng)答機制及相關(guān)疾病的發(fā)生、發(fā)展規(guī)律和臨床治療都有重要意義。細(xì)胞因子的檢測主要分為免疫學(xué)、生物學(xué)和分子生物學(xué)三種檢測方法。 生物學(xué)方法檢測細(xì)胞因子活性,其原理時根據(jù)細(xì)胞因子對特定的依賴性細(xì)胞株(即靶細(xì)胞)的促增值作用,以增值細(xì)胞中的DNA合成或者酶活性作為指標(biāo),間接推算出細(xì)胞因子的生物學(xué)活性。 三、試劑與器材: 10%FCS-RPMI-1640培養(yǎng)液,刀豆蛋白A(ConA),3H-TdR,C57BL小鼠,IL-2標(biāo)準(zhǔn)品,PHA。 離心機、超凈臺、分光光度儀、細(xì)胞培養(yǎng)板等。 四、操作方法: 1.人外周血T細(xì)胞PBMC分離 2.PHA刺激產(chǎn)生IL-2 3.離心去沉淀回收上清 4.IL-2活性測定 5.CTLL-2細(xì)胞株培養(yǎng) 6.MTT摻入 7.測OD值570nm 8.結(jié)果分析 五、關(guān)鍵步驟與注意事項: 1.CTLL-2細(xì)胞存活率應(yīng)大于95%。 2.因標(biāo)本中含IL-4等,可影響TL-2的測定,采用抗IL-4 mAb吸附劑出去IL-4。 3.整個操作過程中應(yīng)該注意無菌操作。 4.細(xì)胞培養(yǎng)時要做復(fù)孔并設(shè)陰、陽性對照。 六、思考題: 1.誘導(dǎo)產(chǎn)生IL-2時需要注意哪些方面? 2.IL-4會對IL-2的測定產(chǎn)生影響,應(yīng)如何消除? 3.PHA誘導(dǎo)產(chǎn)生IL-2的機理是什么?

          乾思 生物elisa試劑盒網(wǎng)發(fā)布

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