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        1. 上海乾思生物科技有限公司
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          使用移液器問(wèn)題解答

          時(shí)間:2011/10/13閱讀:678
          分享:

           乾思 生物編輯整理:

          使用移液器問(wèn)題一:
          移液器滲漏,多道移液器吸嘴里不均衡的液體水平
           
          可能原因
          ·吸嘴與移液器吸嘴基部不配套
          ·當(dāng)裝吸嘴在多道移液器上時(shí)吸嘴架彎曲
          ·吸嘴接觸不緊密
           
          提議
          ·換成質(zhì)量一致的吸嘴(例如: Expel PlusTM 吸嘴)
          ·換成更堅(jiān)固的吸嘴架
          ·稍用力擠壓吸嘴(然而不要用力過(guò)量)
           
          使用移液器問(wèn)題二:
          不準(zhǔn)確性,不性
           
          可能原因
          ·移液器校準(zhǔn)不正確
          ·吸嘴質(zhì)量不夠高
          ·移液器基部的 O 型環(huán)與吸嘴內(nèi)環(huán)沖突
          ·吸嘴基部的 O 型環(huán)用舊了或損壞了
          ·活塞密封(內(nèi)部 O 型環(huán))損壞或用舊了
          ·吸嘴基部可能松了
          ·體積控制器的頂部旋可能松了
           
          提議
          ·根據(jù)說(shuō)明書(shū)重新校準(zhǔn)
          ·換成質(zhì)量一致的吸嘴(例如: Expel PlusTM 吸嘴)
          ·換成沒(méi)有內(nèi)部密封環(huán)的吸嘴
          ·換 O 型環(huán)
          ·卸下吸嘴基部,換 O 型環(huán),檢查校準(zhǔn)
          ·拉緊吸嘴基部
          ·檢查旋鈕,上緊且必要時(shí)重新校準(zhǔn)
           
          使用移液器問(wèn)題三:
          液體殘留在吸嘴里
           
          可能原因
          ·吸嘴可能是親水性的
           
          提議
          ·使用疏水性(低保持力)吸嘴,例如: Expel PlusTM 吸嘴
           
          使用移液器問(wèn)題四:
          液體殘留在吸嘴里
           
          可能原因
          ·活塞密封(內(nèi)部 O 型環(huán))損壞或用舊了
          ·分散于活塞上的油不均衡(可能由儲(chǔ)存過(guò)長(zhǎng),沒(méi)有垂直儲(chǔ)存移液器或在中間體積過(guò)度使用移液器等引起)
          ·活塞需要潤(rùn)滑油
          ·活塞上具有堆積物
           
          提議
          ·卸下吸嘴基部,換 O 型環(huán),檢查校準(zhǔn)
          ·將移液器調(diào)至zui大體積(吸嘴:使用移液器隨機(jī)贈(zèng)送的固定體積移液器),使活塞充分上下來(lái)回 3-5 次
          ·根據(jù)說(shuō)明書(shū)潤(rùn)滑活塞
          ·清洗活塞并根據(jù)說(shuō)明書(shū)重新潤(rùn)滑
           
           
          乾思 生物提醒:
          ·總是以直立(垂直)狀態(tài)儲(chǔ)存移液器。每天早晨通過(guò)在zui大體積處壓下活塞 4-5 次,預(yù)先準(zhǔn)備好移液器
          ·千萬(wàn)不要將帶有吸嘴(里面有液體)的移液器倒置或水平放置??偸钦_的裝置高質(zhì)量的吸嘴。

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