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        1. 上海乾思生物科技有限公司
          中級會員 | 第16年

          18916423352

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          SIGMA現(xiàn)貨,SIGMA代理

          移液器的檢測和校準(zhǔn)指南

          時(shí)間:2011/10/13閱讀:540
          分享:
          乾思 生物編輯整理:
          一、實(shí)驗(yàn)室簡單檢測
          (一)氣密性檢測
          移液器吸滿液體后,手持垂直放置15s,檢查吸嘴的尖頭有無液滴,如有,則說明漏氣。
          (二)準(zhǔn)確性檢測
          1)量程小于1μl,建議使用分光光度法檢測。將移液器調(diào)至目標(biāo)體積,然后移取染料溶液,加入一定體積的蒸餾水中,測定溶液的稀釋度(334nm或340nm),重復(fù)幾次移液操作,計(jì)算移液器的度。
          2)量程大于1μl,可以用稱重法檢測。通過對水的稱重,轉(zhuǎn)換成體積(體積=質(zhì)量/密度),鑒別移液器的準(zhǔn)確性。由于水的密度是隨著溫度變化而變化,且稱量天平本身度不符合檢測要求,檢測又大多在一個開放式空間內(nèi)操作,偏差在所難免。因而,此種稱量法只能現(xiàn)場粗略地判斷移液器的準(zhǔn)確性,進(jìn)一步的校準(zhǔn)必須在專業(yè)的實(shí)驗(yàn)室操作進(jìn)行。
          注意:稱重法實(shí)驗(yàn)室*條件是高度靈敏的分析天平(定期校準(zhǔn))、雙蒸水和稱量容器。水、移液器和吸嘴必須具有相同的溫度(20℃時(shí)水的密度為0.09982)。
          二、專業(yè)校準(zhǔn)
          移液器的工作量大,長期使用,將會使彈簧彈力發(fā)生變形,加之本身是塑料,不耐磨擦,就會產(chǎn)生誤差。為了保證移液器傳遞液體量的準(zhǔn)確性,必須對移液器進(jìn)行定期校準(zhǔn)。為了更好地發(fā)揮該類計(jì)量儀器的作用,對新購進(jìn)的儀器要進(jìn)行校準(zhǔn)才能使用;對正在使用的計(jì)量儀器,由于長期使用會造成其示值于度量對象的誤差,這種誤差若不進(jìn)行控制,及時(shí)校正,必定會影響科研工作。因此,對使用的移液器要定期檢定、校正,并建立檔案,只有這樣才能使其在日常工作中更好的發(fā)揮作用。

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