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        1. 上海乾思生物科技有限公司
          中級會員 | 第16年

          18916423352

          細胞庫
          優(yōu)勢品牌
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          自產(chǎn)ELISA試劑盒,試劑盒
          進口ELISA試劑盒
          人細胞,人細胞系,人細胞株
          3h biomedical,3H Biomedical AB,3hbiomedical,3-M Company,4adi,4rtilab,5 prime,5prime a & g pharmaceuticals,A&D Weighing,Aalto Scientific,Aalto Scientific ltd,Aalto Scientific,ltd.,aaltobioreagents,aaltoscientific,aat bioquest AAT Bioquest Inc,AB Biolabs,ab peptides,AB Sciex,Abaxis,Abazyme,Abberior,abbexa abbiotec,Abbkine,abbomax,Abbott Molecular,abcam,ABclonal,ABclonal Inc.,abcore-inc abcr GmbH,abcrGmbH,abcysonline,ABD,ABD Bioquest,Inc,abd serotec,ab-direct,abdserotec Abeomics,abfrontier,abgab,abgene,abgent,ABI Applied Biosystems,abiocode,abionline Abkem Iberia,ABL,Inc,Abliance,abm Inc.,abmart,abmgood,Abmole,Abmole Bioscience Inc. abnova,Abraxis,Absolute Antibody,ABT Beads,abt beads technologies,ABTbeads,abvision,Abways abzoom,academy biomed,academy bio-medical,acanthus research,acanthusresearch,ACC,accelagen,Accelagen inc. Access Biologicals,LLC,accessbiologicals,acciusa,accu-chek,AccuDiag Allergy,AccuDiag Anemia,AccuDiag Autoimmune Diseases,AccuDiag Bone Metabolism AccuDiag Thyroid,accumax accutase,Accumetrics,Accurate,accurate chemical,Accurate Wirecraft Company,accuratechemical,accustandard acdbio,Ace Glass,Ace Hose and Rubber Co,ACEA Biosciences,ACL Incorporated,ACME Microspheres Inc,ACMEmicrospheres,acris acris antibodies,Acris Antibodies GmbH,acris-antibodies,ACROBiosystems,acrometrix,acros,Acros Organics,ACT Test Panels LLC actc,actgene,active motif,activemotif,Adam Equipment,Inc,AddexBio,Addexbio Technologies,addgene ademtech,adg,ADI,ADInstruments,adipogen,Adolf Kuhner,adooq,Advance Tabco advanced immunochemical,advanced immunochemical inc,advanced targeting systems,Advansta,Advansta Inc.,Advansta,Inc,Advantech,Advent Electric Inc adventbio,advnt,advtechind,advy chemical,advy chemical Purified Proteins,advychemical Polyclonal Antisera,aesku,aetltd affiland,affinity,affinity biologicals,affinity bioreagent,Affinity BioReagents,Affinity BioReagents abr,Affinity Biosciences,affinitybiologicals AffinityBioReagents,AffinityImmuno,affix scientific,affixscientific,affymetrix,ag scientific,Agar Scientific,agarscientific agdia,Agela Technologies,agilent,Agilent Seahorse,agilent technologies,agilet,agilet solutions,agiletsolutions agrenvec,agrisera,AGRO-BIO,agscientific,ahdiagnostics,ahf,AHF analysentechnik,AHF analysentechnik AG Air Technology Solutions,Airgas Safety,Air-Tite,AJAX SCIENTIFIC LTD,AK Limited,Akro-Mils,ALA Scientfic,alamarblue Alba Bioscience,Alconox Inc,aldevron,alerchek,alere,Alexis,ALEXIS Biochemicals,Alfa Aesar Aline Biosciences,AlineBiosciences,All Foils Incorporated,Allcells,allele biotech,allelebiotech,allen-bradley,AllerMAbs
          大鼠細胞,大鼠細胞系
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          SIGMA現(xiàn)貨,SIGMA代理

          培養(yǎng)基的滅菌原理及方法

          時間:2011/10/26閱讀:841
          分享:

           所謂滅菌就是殺死一切微生物,包括微生物的營養(yǎng)體和芽孢,這一概念不同于消毒。后者是指消滅一切致病微生物(病原體)。在發(fā)酵生產(chǎn)中為什么要滅菌呢?主要有以下幾點原因:如不滅菌,會使生物反應的基質(zhì)或產(chǎn)物,因雜菌的消耗而損失,造成生產(chǎn)能力的下降;雜菌也會產(chǎn)生代謝產(chǎn)物,這就使產(chǎn)物的提取更加困難,造成得率降低,產(chǎn)品質(zhì)量下降;有些雜菌會分解產(chǎn)物,使生產(chǎn)失??;雜菌大量繁殖后,會改變反應液的pH值,使反應異常;如果發(fā)生噬菌體污染,生產(chǎn)菌細胞將被裂解,使生產(chǎn)失敗。一、培養(yǎng)基滅菌的原理和方法:滅菌的方法很多,在實驗室可以使用干熱滅菌、對于環(huán)境可以使用化學試劑滅菌,但化學試劑的滅菌方法有很大的限制。在工業(yè)生產(chǎn)中,對于培養(yǎng)基、管道、設備的滅菌,通常采用蒸汽加熱到一定的溫度,并保溫一段時間的滅菌方法,稱之為濕熱滅菌。濕熱滅菌的顯著優(yōu)點是:使用方便,無污染,而且其冷凝水可以直接冷凝在培養(yǎng)基中,也可以通過管道排出。滅菌的原理在這里先講幾個概念:①致死溫度:殺死微生物的極限溫度。②致死時間:在致死溫度下,殺死全部微生物所需要的時間。③微生物的熱阻:表示微生物對熱的抵抗能力,即指微生物在某一特定條件下(主要是溫度)的致死時間。其對熱的抵抗能力越大,可以理解為熱阻越大,衡量不同的微生物對熱的抵抗能力的大小,可以使用相對熱阻的概念。④相對熱阻:某一微生物在某一特定條件下的致死時間與另一微生物在相同條件下的致死時間之比。例如:芽孢/大腸桿菌=3000000/1;病毒/大腸桿菌=1—5/1等。

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