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        1. 上海乾思生物科技有限公司
          中級(jí)會(huì)員 | 第16年

          18916423352

          細(xì)胞庫(kù)
          優(yōu)勢(shì)品牌
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          自產(chǎn)ELISA試劑盒,試劑盒
          進(jìn)口ELISA試劑盒
          人細(xì)胞,人細(xì)胞系,人細(xì)胞株
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          大鼠細(xì)胞,大鼠細(xì)胞系
          細(xì)胞株,細(xì)胞系,傳代細(xì)胞
          白鰱細(xì)胞 斑馬魚(yú)細(xì)胞 豹貓細(xì)胞 成人細(xì)胞 赤狐細(xì)胞 大額牛細(xì)胞 大耳山羊細(xì)胞 大黃魚(yú)細(xì)胞 大長(zhǎng)舌果蝠細(xì)胞 俄勒岡地鼠細(xì)胞 非洲綠猴細(xì)胞 高背鯽魚(yú)細(xì)胞 果蠅細(xì)胞 果子貍細(xì)胞 荷蘭黑白花奶牛細(xì)胞 黑化大家鼠細(xì)胞 花鼠細(xì)胞 家兔細(xì)胞 昆蟲(chóng)細(xì)胞 綠猴細(xì)胞 馬鐵菊頭蝠細(xì)胞 綿羊細(xì)胞 三葉小蹄蝠細(xì)胞 散鱗鏡鯉細(xì)胞 社鼠細(xì)胞 鼠細(xì)胞 樹(shù)鼩細(xì)胞 團(tuán)頭魴細(xì)胞 新生兒細(xì)胞 新生牛細(xì)胞 敘利亞倉(cāng)鼠細(xì)胞 長(zhǎng)尾綠猴細(xì)胞 中國(guó)倉(cāng)鼠細(xì)胞 中華鱉細(xì)胞 棕果蝠細(xì)胞 腫瘤細(xì)胞 豬細(xì)胞 兔細(xì)胞 犬細(xì)胞
          抗體庫(kù),小包裝,流式抗體
          SIGMA現(xiàn)貨,SIGMA代理

          近年來(lái)新建立的與補(bǔ)體有關(guān)的血清學(xué)試驗(yàn)

          時(shí)間:2011/10/26閱讀:591
          分享:
          (一) 單擴(kuò)散溶血試驗(yàn)(single radial hemolysis) 又稱固相溶血試驗(yàn)。根據(jù)抗原(如綿羊紅細(xì)胞)與相應(yīng)抗體相遇形成的免疫復(fù)合物,可通過(guò)傳統(tǒng)途徑激活補(bǔ)體,使綿羊紅細(xì)胞發(fā)生溶血,其溶血程度與特異抗體滴度成正比的原理設(shè)計(jì)而成?,F(xiàn)以鼻疽為例說(shuō)明其操作原則。取鼻疽抗原致敏的綿羊紅細(xì)胞加入融化的瓊脂糖凝膠中,混合均勻,澆注反應(yīng)盤(pán),制成致敏的反應(yīng)盤(pán),打孔,隨后將被檢血清和補(bǔ)體的等量混合物加入到上述致敏反應(yīng)盤(pán)孔中,于室溫下感作3~4小時(shí),陽(yáng)性者,在孔的周圍發(fā)生明顯的溶血環(huán),其直徑在8.1mm以上。(二) 固相補(bǔ)體結(jié)合試驗(yàn)(solid phase CFT) 系根據(jù)直接補(bǔ)體結(jié)合反應(yīng)的原理設(shè)計(jì)而成,只不過(guò)用瓊脂糖凝膠代替生理鹽水作介質(zhì)實(shí)施反應(yīng),即將鼻疽抗原、被檢血清與補(bǔ)體等量混合,放恒溫箱中感作1小時(shí)后,取混合物滴加于預(yù)先制備好的含溶血素致敏綿羊紅細(xì)胞的瓊脂糖凝膠的相應(yīng)孔中,與此同時(shí),每份血清必須作一個(gè)不加抗原的對(duì)照孔,再置恒溫箱中感作3小時(shí),然后根據(jù)每份血清對(duì)照孔與試驗(yàn)孔之間發(fā)生的溶血環(huán)直接差的大小,確定檢驗(yàn)結(jié)果,凡在0.6mm以上者,判為鼻疽陽(yáng)性。

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