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        1. 上海乾思生物科技有限公司
          中級(jí)會(huì)員 | 第16年

          18916423352

          細(xì)胞庫(kù)
          優(yōu)勢(shì)品牌
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          自產(chǎn)ELISA試劑盒,試劑盒
          進(jìn)口ELISA試劑盒
          人細(xì)胞,人細(xì)胞系,人細(xì)胞株
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          大鼠細(xì)胞,大鼠細(xì)胞系
          細(xì)胞株,細(xì)胞系,傳代細(xì)胞
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          抗體庫(kù),小包裝,流式抗體
          SIGMA現(xiàn)貨,SIGMA代理

          沙門(mén)氏菌培養(yǎng)基的配置過(guò)程

          時(shí)間:2011/11/7閱讀:550
          分享:

           名稱(chēng):沙門(mén)氏菌培養(yǎng)基

          用 途:用于腸道致病菌特別是沙門(mén)氏菌和志賀氏菌的選擇性分離培養(yǎng)
          原 理:蛋白胨、牛肉膏粉提供碳源、氮源、維生素和礦物質(zhì);乳糖、蔗糖為可發(fā)酵的糖類(lèi);去氧膽酸鈉和檸檬酸鈉抑制革蘭氏陽(yáng)性菌及大多數(shù)的大腸菌群和變形桿菌,但不影響沙門(mén)氏菌的生長(zhǎng);硫代硫酸鈉和檸檬酸鐵銨用于檢測(cè)硫化氫的產(chǎn)生,使菌落中心呈黑色;中性紅為ph指示劑,發(fā)酵糖產(chǎn)酸的菌落呈紅色,不發(fā)酵糖的菌落為無(wú)色;瓊脂是培養(yǎng)基的凝固劑。
          配方(每升):胨 20.0g乳糖 10.0g牛肉膏粉3.0g蔗糖 10.0g去氧膽酸鈉 1.0g硫代硫酸鈉2.3g檸檬酸鈉 1.0g檸檬酸鐵銨1.0g中性紅 0.03g瓊脂 16.0gzui終ph 7.2±0.1
          使用方法:
          1.稱(chēng)取本品63.3g,加入蒸餾水或去離子水1 l,攪拌加熱煮沸至*溶解,待冷至50℃左右,在無(wú)菌環(huán)境中,傾注滅菌平皿,待凝固后,備用。
          2.用接種環(huán)取增菌液一環(huán),劃線接種于平板上。
          3.將平板放入恒溫培養(yǎng)箱中,36±1℃培養(yǎng)18~24h。
          4.觀察結(jié)果。

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