在2017年11月11日，由Fanni Li，Wenxi Dong，Peng Lin和Jianli Jiang在Biology of Reproduction中發(fā)表一篇了《Regulation of Akt/FoxO3a/Skp2 Axis Is Critically Involved in Berberine-Induced Cell Cycle Arrest in Hepatocellular Carcinoma Cells》的文章。

文章使用ECHO Revolve正倒置一體顯微鏡獲得了如下結(jié)果:

中文摘要：

ECHO Revolve正倒置一體顯微鏡觀察到的有序的細(xì)胞周期是腫瘤發(fā)生中關(guān)鍵的生物學(xué)過(guò)程，是抗癌藥物的重要靶點(diǎn)。小檗堿（BBR）是一種重要的天然異喹啉類(lèi)生物堿，具有抗癌潛能，可誘導(dǎo)肝癌（HCC）細(xì)胞周期阻滯。然而，其基本機(jī)制尚待闡明。在我們目前研究中，觀察到小檗堿處理的HUH-7和HepG2細(xì)胞G0/G1細(xì)胞周期阻滯。簡(jiǎn)單的說(shuō)，BBR可使AKT通路失活，從而抑制S期激酶相關(guān)蛋白SKP2的表達(dá)，并增強(qiáng)FoxO3a在細(xì)胞核中的表達(dá)和轉(zhuǎn)運(yùn)。易位的FXO3a一方面能直接促進(jìn)細(xì)胞周期蛋白依賴性激酶抑制劑（CDKIS）p21^CIP1和p27^kip1的轉(zhuǎn)錄，另一方面抑制Skp2的表達(dá)，導(dǎo)致p21^CIP1和p27^kip1上調(diào)，肝癌HCC G0/G1細(xì)胞周期阻滯。綜上所述，BBR通過(guò)調(diào)節(jié)Akt/FoxO3A/Skp2促進(jìn)CDKIs p21^CIP1和p27^kip1的表達(dá)，誘導(dǎo)肝癌HCC G0/G1細(xì)胞周期阻滯。本研究揭示了BBR抗癌作用的新機(jī)制。

英文摘要：

The maintenance of ordinal cell cycle phases is a critical biological process in cancer genesis, which is a crucial target for anti-cancer drugs. As an important natural isoquinoline alkaloid from Chinese herbal medicine, Berberine (BBR) has been reported to possess anti-cancer potentiality to

induce cell cycle arrest in hepatocellular carcinoma cells (HCC). However, the underlying mechanism remains to be elucidated. In our present study, G0/G1 phase cell cycle arrest was observed in berberine-treated Huh-7 and HepG2 cells. Mechanically, we observed that BBR could deactivate the Akt pathway, which consequently suppressed the S-phase kinase-associated protein 2 (Skp2) expression and enhanced the expression and translocation of Forkhead box O3a (FoxO3a) into nucleus. The translocated FoxO3a on one hand could directly promote the transcription of cyclin-dependent kinase inhibitors (CDKIs) p21^Cip1 and p27^Kip1, on the other hand, it could repress Skp2 expression, both of which lead to up-regulation of p21^Cip1 and p27^Kip1, causing G0/G1 phase cell cycle arrest in HCC. In conclusion, BBR promotes the expression of CDKIs p21^Cip1 and p27^Kip1 via regulating the Akt/FoxO3a/Skp2 axis and further induces HCC G0/G1 phase cell cycle arrest. This research uncovered a new mechanism of an anti-cancer effect of BBR. 

ECHO Revolve正倒置一體顯微鏡展現(xiàn)了其非凡的靈活性，可以輕松地實(shí)現(xiàn)正置和倒置顯微鏡轉(zhuǎn)換，創(chuàng)新性地把正倒置顯微鏡合二為一，開(kāi)啟了顯微鏡Hybrid時(shí)代。

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ECHO Revolve正倒置一體顯微鏡兼具正置和倒置顯微鏡的優(yōu)勢(shì)，方便小巧，一機(jī)多能，可以非常便利地通過(guò)旋轉(zhuǎn)實(shí)現(xiàn)正倒置配置切換；以iPad桌面替代目鏡設(shè)計(jì), 獲得的視覺(jué)體驗(yàn)；同時(shí)基于iOS的Echo app，使軟件操作更人性化。