在2017年12月27日，由Jing Xu，F(xiàn)uhua Xu，Maralee S Lawson，Olena Y Tkachenko等作者在Biology of Reproduction中發(fā)表一篇了《Anti-Mullerian hormone is a survival factor and promotes the growth of rhesusmacaque preantral follicles during matrix-free culture》的文章。

文章使用Revolve正倒置一體顯微鏡獲得了如下結(jié)果:

該圖中B-E是通過Revolve正倒置一體顯微鏡成像得到的結(jié)果 ↑

該圖中A-B、C-F是通過Revolve正倒置一體顯微鏡成像得到的結(jié)果 ↑

中文摘要：

抗穆勒氏管激素（AMH）在卵泡發(fā)育過程起著至關(guān)重要的作用，其局部作用于卵泡生長(zhǎng)過程中的動(dòng)態(tài)分泌有關(guān)。目前不同物種的AMH在腔內(nèi)卵泡生長(zhǎng)和功能的影響已有所驗(yàn)證，但對(duì)腔前卵泡發(fā)育的作用仍待進(jìn)一步驗(yàn)證。因此，我們通過實(shí)驗(yàn)研究AMH在腔前卵泡發(fā)育過程中所起到的作用。利用AMH-shRNA重組腺病毒載體降低獼猴卵泡中AMH基因的表達(dá)。將次生卵泡從成年獼猴卵巢中分離出來后用超低附著培養(yǎng)皿單獨(dú)培養(yǎng)，且培養(yǎng)基中加入促卵泡激素和胰島素，培養(yǎng)5周。將卵泡隨機(jī)分配給治療組，分別為a.對(duì)照組；b.非靶向?qū)φ战MshRNA-vector；c.AMH shRNA-vector；d.AMH shRNA-vector + 重組人源AMH；e.重組人源AMH。評(píng)估卵泡的存活和生長(zhǎng)情況，并分析培養(yǎng)基中的類固醇激素和旁分泌因子的濃度。在體內(nèi)研究中，將非靶向?qū)φ誷hRNA-vecotor和AMH shRNA-vector分別注入獼猴的卵巢，術(shù)后9天對(duì)卵巢進(jìn)行形態(tài)學(xué)及免疫組化檢測(cè)。結(jié)果發(fā)現(xiàn)，在非人靈長(zhǎng)類動(dòng)物中，AMH表達(dá)下調(diào)抑制腔前卵泡的存活和生長(zhǎng)。體外實(shí)驗(yàn)發(fā)現(xiàn)，在培養(yǎng)基中添加AMH則可促進(jìn)腔前卵泡生長(zhǎng)發(fā)育至小腔竇期，類固醇激素和旁分泌因子表達(dá)增加，并促進(jìn)卵母細(xì)胞成熟。這些數(shù)據(jù)表明，AMH是一個(gè)關(guān)鍵的卵泡旁分泌/自分泌因子，促進(jìn)靈長(zhǎng)類的腔前卵泡存活和生長(zhǎng)。

英文摘要：

Anti-Mullerian hormone (AMH) plays a key role during ovarian follicular development, with local actions associated with a dynamic secretion profile by growing follicles. While results for AMH effects on antral follicle growth and function are consistent among studies in various species, any effects on preantral follicle development remain controversial. Therefore, experiments were conducted to investigate the direct actions and role of AMH during follicle development at the preantral stage. Macaque-specific short-hairpin RNAs (shRNAs) targeting AMH mRNA were incor- porated into adenoviral vectors to decrease AMH gene expression in rhesus macaque follicles. Secondary follicles were isolated from adult macaque ovaries and cultured individually in the ultra-low-attachment dish containing defined medium supplemented with follicle-stimulating hor- mone and insulin for 5 weeks. Follicles were randomly assigned to treatment groups: (a) control,(b) nontargeting control shRNA-vector, (c) AMH shRNA-vector, (d) AMH shRNA-vector + recombi-nant human AMH, and (e) recombinant human AMH. Follicle survival and growth were assessed. Culture media were analyzed for steroid hormone and paracrine factor concentrations. For in vivo study, the nontargeting control shRNA-vector and AMH shRNA-vector were injected into macaque ovaries. Ovaries were collected 9 days postinjection for morphology and immunohistochemistry assessment. Decreased AMH expression reduced preantral follicle survival and growth in non- human primates. Supplemental AMH treatment in the culture media promoted preantral follicle growth to the small antral stage in vitro with increased steroid hormone and paracrine factor production, as well as oocyte maturation. These data demonstrate that AMH is a critical follicular paracrine/autocrine factor positively impacting preantral follicle survival and growth in primates.

ECHO Revolve正倒置一體顯微鏡展現(xiàn)了其非凡的靈活性，可以輕松地實(shí)現(xiàn)正置和倒置顯微鏡轉(zhuǎn)換，創(chuàng)新性地把正倒置顯微鏡合二為一，開啟了顯微鏡Hybrid時(shí)代。

>   視網(wǎng)膜屏顯示技術(shù)---比擬目鏡人眼觀察效果

>   全視野觀察---更清晰，更方便

>   多通道熒光---多達(dá)4個(gè)EPI熒光通道，通過iPad Pro實(shí)現(xiàn)了*自動(dòng)化操作，輕松快速地完成多色熒光顯微分析

>   App應(yīng)用軟件---基于IOS的Echo App是與Apple團(tuán)隊(duì)合作研發(fā)的專業(yè)顯微鏡軟件

>   的工藝盡顯品質(zhì)---實(shí)現(xiàn)非凡的性能

ECHO Revolve正倒置一體顯微鏡兼具正置和倒置顯微鏡的優(yōu)勢(shì)，方便小巧，一機(jī)多能，可以非常便利地通過旋轉(zhuǎn)實(shí)現(xiàn)正倒置配置切換；以iPad桌面替代目鏡設(shè)計(jì), 獲得的視覺體驗(yàn)；同時(shí)基于iOS的Echo app，使軟件操作更人性化。