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        1. 上海易佰聚經(jīng)貿(mào)有限公司
          中級會(huì)員 | 第15年

          13636495876

          當(dāng)前位置:上海易佰聚經(jīng)貿(mào)有限公司>>生化試劑>>瓊脂糖>> 22585PIERCE,DITHIOBIS (SUCCINIMIDYL PROPIONATE),(DSP)

          PIERCE,DITHIOBIS (SUCCINIMIDYL PROPIONATE),(DSP)

          參  考  價(jià)面議
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          產(chǎn)品型號22585

          品       牌PIERCE

          廠商性質(zhì)經(jīng)銷商

          所  在  地上海市

          更新時(shí)間:2024-05-31 12:03:32瀏覽次數(shù):1846次

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          貨號:22585,名稱: DITHIOBIS (SUCCINIMIDYL PROPIONATE),(DSP) ,規(guī)格:1 GM

          訂貨:,


          DITHIOBIS (SUCCINIMIDYL PROPIONATE),(DSP)介紹


          Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。


          DITHIOBIS (SUCCINIMIDYL PROPIONATE)(DSP)現(xiàn)貨供應(yīng)



          PIERCE

          22585

          DITHIOBIS (SUCCINIMIDYL PROPIONATE),(DSP)         1 GM

          1 GM

          PIERCE

          22586

          DSP (Lomant's Reagent), 50 mg

          50 MG

          PIERCE

          22589

          (SULFO-SANPAH) SULFOSUCCINIMIDYL 6-(4'-AZIDO-2'-) NITROPHENY

          50 MG


          DITHIOBIS (SUCCINIMIDYL PROPIONATE),(DSP)品牌介紹


          美國Pierce公司(www.pierce.com)是蛋白質(zhì)化學(xué)、免疫學(xué)和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

           

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          DITHIOBIS (SUCCINIMIDYL PROPIONATE)(DSP)

          僅用于科研


          Thermo Scientific Pierce DSP (Lomant's Reagent) is an amine-reactive crosslinker having NHS-ester reactive ends and a cleavable disulfide bond in its spacer arm.

          DSP contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxy-succinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents. DSS, the non-cleavable analog of DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

          DSP Highlights

          • Amine-reactive NHS ester reacts rapidly with any primary amine-containing molecule
          • Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
          • Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
          • Cleavable crosslinker allows separation of crosslinked products
          • DSP crosslinker is membrane permeable, so intracellular crosslinking can be done
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