溶壁微球菌
Micrococcus lysodeikticus Cells
1、培養(yǎng)基:營(yíng)養(yǎng)肉汁瓊脂
Pep (蒸餾水)1000ml Adjust(調(diào))pH to 7.0-7.2
溶壁微球菌
(注意):培養(yǎng)芽孢桿菌時(shí)加入5毫克MnSO4. H2O,則有利于產(chǎn)生芽孢。ton(蛋白胨) 5g Beef extract 3g
NaCl 5g Agar (瓊脂) 20g
Distilled water
2、培養(yǎng)溫度: 30℃
3、培養(yǎng)時(shí)間: 24-48h
溶壁微球菌
1、微生物菌種應(yīng)保藏于低溫、清潔和干燥的地方,室溫放置時(shí)間過長(zhǎng)會(huì)導(dǎo)致菌種衰退;
2、菌種操作應(yīng)在無菌條件下進(jìn)行,防止雜菌污染;
3、斜面菌種保藏時(shí)間通常為3-6個(gè)月,應(yīng)根據(jù)菌種狀況及時(shí)轉(zhuǎn)接;凍干菌種保藏時(shí)間通常為2-15年;
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