CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和*培養(yǎng)條件!
CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞 的詳細(xì)介紹
CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞
| ATCC? Number: | CRL-6475? | Price: | $269.00 |
| Designations: | B16-F10 | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Mus musculus （mouse） | | Morphology: | melanocyte
 | | Source: | Organ: skin
Strain: C57BL/6J
Disease: melanoma | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| | Applications: | transfection host （technology from amaxa） | | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide （CO2）, 5% | | Subculturing: | Protocol: - Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
- Subc*tion Ratio: A subc*tion ratio of 1:10 is recommended
Medium Renewal: Every 2 to 3 days
| | Preservation: | Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
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