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        1. 上海復(fù)祥生物科技有限公司

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          [供應(yīng)]CRL-1646 FO 小鼠骨髓瘤細胞

          貨物所在地:上海上海市

          更新時間:2024-10-28 21:00:07

          有效期:2024年10月28日 -- 2025年4月28日

          已獲點擊:200

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          CRL-1646 FO 小鼠骨髓瘤細胞, ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件
          CRL-1646 FO 小鼠骨髓瘤細胞 的詳細介紹

          CRL-1646 FO 小鼠骨髓瘤細胞

          ATCC® Number:  CRL-1646™      Price:  $355.00 
          Designations:  FO 
          Depositors:   SF de St. Groth 
          Biosafety Level: 1 
          Shipped:  frozen 
          Medium & Serum:  See Propagation 
          Growth Properties: suspension
          Organism: Mus musculus (mouse) 
          Morphology: lymphoblast

           
          Source: Disease: plasmacytoma; myeloma
          Strain: BALB/c
          Cell Type: B lymphoblast;
          Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
           
          Antigen Expression: H-2d
          Comments: The line was line created by self fusing Sp2/0 myeloma cells using Sendai virus.
          The cells are resistant to 8-azaguanine and do not produce immunoglobulin.
          The line can be used a fusion partner for making B cell hybridomas.
          Tested and found negative for ectromelia virus (mousepox).
          Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
          Temperature: 37.0°C
          Atmosphere: air, 95%; carbon dioxide (CO2), 5%
          Subculturing:  Medium Renewal: Every 2 to 3 days
          Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 to 1 X 10 exp5 viable cells/ml.
          Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
          Preservation:  culture medium 95%; DMSO, 5%
          Doubling Time:  8 to 10 hrs 
          Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
          recommended serum:ATCC 30-2020
          References: 1092: de St.Groth SF, Scheidegger D. Production of monoclonal antibodies: strategy and tactics. J. Immunol. Methods 35: 1-21, 1980. PubMed: 7009747
           Related Links  
          NCBI Entrez Search 
          Make a Deposit 
          Frequently Asked Questions 
          Material Transfer Agreement 
          Technical Support 
          Related Cell Culture Products  
           
           

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