CCL-46 P388D1 小鼠淋巴樣瘤細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和*培養(yǎng)條件!
CCL-46 P388D1 小鼠淋巴樣瘤細(xì)胞 的詳細(xì)介紹
CCL-46 P388D1 小鼠淋巴樣瘤細(xì)胞
ATCC® Number: CCL-46™
Designations: P388D1
Depositors: EM Jensen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus （mouse）
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: DBA/2
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: Biological response [92560]
transfection host （Roche FuGENE® Transfection Reagents）
Tumorigenic: Yes
Reverse Transcript: positive
Antigen Expression: H-2, d haplotype; Mus musculus, expressed
Comments: A subclone of this line [see ATCC TIB-63, P388D1（IL-1）] produces high levels of interleukin-1 （IL-1, interleukin 1）.Tested and found negative for ectromelia virus （mousepox）.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 - 5 X 10 （5） viable cells/ml.
A maximum of 6 X 10 （6） viable cells/ml is obtainable.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 10 to 12 hrs
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002
recommended serum:ATCC 30-2040
Cell culture tested DMSO:ATCC 4-X
References: 22208: . . Am. J. Pathol. 33: 603, 1957.
92560: Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.
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