CRL-5803 NCI-H1299 人非小細(xì)胞肺癌細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫(kù)管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和*培養(yǎng)條件!
CRL-5803 NCI-H1299 人非小細(xì)胞肺癌細(xì)胞
的詳細(xì)介紹
CRL-5803 NCI-H1299 人非小細(xì)胞肺癌細(xì)胞
| ATCC® Number: | CRL-5803™ | | |
| Designations: | NCI-H1299 | | Depositors: | AF Gazdar, JD Minna | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Homo sapiens (human) | | Morphology: | epithelial
| | Source: | Organ: lung
Disease: carcinoma; non-small cell lung cancer
Derived from metastatic site: lymph node | | Cellular Products: | neuromedin B | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | | | Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. ephone (214) 699-8056, (214) 688-7233. | | Applications: | transfection host (Roche Transfection Reagents) | | DNA Profile (STR): | Amelogenin:X
CSF1PO:12
D13S317:12
D16S539:12,13
D5S818:11
D7S820:10
THO1:6,9.3
TPOX:8
vWA: 16,17,18 | | Age: | 43 years adult | | Gender: | male | | Ethnicity: | Caucasian | | Comments: | The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. They reported to be able to synthesize the peptide neuromedin B (NMB) at 0.1 pmol/mg protein, but not the gastrin releasing peptide (GRP). | | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% | | Subculturing: | Protocol:- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37C. CRL-5803 NCI-H1299
- Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
| | Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase | | Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
purified DNA:ATCC CRL-5803D | | References: | 23517: Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005
23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..
33177: Lin DL, Chang C. p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. J. Biol. Chem. 271: 14649-14652, 1996. PubMed: 8663350 CRL-5803 NCI-H1299 |
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MLE-12 小鼠肺上皮細(xì)胞
saos-2 人成骨肉瘤細(xì)胞
MA104 非洲綠猴胚胎腎上皮細(xì)胞
ball-1 細(xì)胞
nci-h226 人肺鱗癌細(xì)胞
MCA38 小鼠結(jié)腸癌細(xì)胞
LTPA 小鼠胰腺癌細(xì)胞
NCI-H23
293.00 人胚腎細(xì)胞
CRL-5803 NCI-H1299
293FT 人胚腎細(xì)胞-用于慢病毒包裝
293TN 人胚腎細(xì)胞--用于慢病毒包裝
293AV 人胚腎細(xì)胞-用于腺病毒包裝
293T 人胚腎細(xì)胞
208F 大鼠成纖維細(xì)胞
786-O 人腎透明細(xì)胞腺癌細(xì)胞
A172 人腦膠質(zhì)母細(xì)胞瘤細(xì)胞
A549 人肺癌細(xì)胞 細(xì)胞系購(gòu)買
AGS 人胃癌細(xì)胞
BEL7402 人肝癌細(xì)胞系
AZ-521 人胃癌細(xì)胞
B16F0 小鼠黑色素瘤細(xì)胞
Bel-7402 人肝癌細(xì)胞
Bel-7404 人肝癌細(xì)胞
BGC-823 人胃癌細(xì)胞
C6 大鼠腦膠質(zhì)瘤細(xì)胞
SW480 人結(jié)腸腺癌細(xì)胞
CaCo-2 人結(jié)腸癌細(xì)胞
Cho-K1 倉(cāng)鼠卵巢細(xì)胞亞株
COS-7 非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞
GES-1 人胃上皮細(xì)胞
HCT-8 人回盲腸癌細(xì)胞
HeLa 人宮頸癌細(xì)胞
Hepa1-6 小鼠肝癌細(xì)胞
HepG2 人肝癌細(xì)胞
hFOB1.19 人永生化成骨細(xì)胞
HGC-27 人胃癌細(xì)胞
Hs 746T 人胃癌細(xì)胞
HT-29 人結(jié)腸癌細(xì)胞
KB 人口腔表皮樣癌細(xì)胞
LoVo 人結(jié)腸癌細(xì)胞
LLC-PK1 豬腎近曲小管上皮細(xì)胞
MCF-10A 人正常乳腺上皮細(xì)胞
MCF-7 人乳腺癌細(xì)胞
MDA-MB-231 人乳腺癌細(xì)胞
MGC80-3 人胃癌細(xì)胞
MKN-45 人胃癌細(xì)胞
NCI-H460 人大細(xì)胞肺癌細(xì)胞
NIH-3T3 小鼠成纖維細(xì)胞
NUGC-3 人胃癌細(xì)胞
Raw264-7 小鼠單核細(xì)胞白血病細(xì)胞
SGC-7901 人胃癌細(xì)胞
SMMC-7721 人肝癌細(xì)胞
SNU-638 人胃癌細(xì)胞
SNU-739 人肝癌細(xì)胞
Tca8113 人舌鱗癌細(xì)胞 細(xì)胞系購(gòu)買
U251 人腦膠質(zhì)瘤細(xì)胞
U-373MG 人腦神經(jīng)膠質(zhì)瘤細(xì)胞
U-87 MG 人腦星形膠質(zhì)母細(xì)胞瘤
BT-474 人乳腺導(dǎo)管癌細(xì)胞
PANC-1 人胰腺癌細(xì)胞
HEK293 人胚腎細(xì)胞
Hep3B 人肝癌細(xì)胞
HL-7702 人正常肝細(xì)胞
Raw-Blue 小鼠單核細(xì)胞白血病細(xì)胞
WEHI-231 小鼠B淋巴細(xì)胞
OPM-2 人骨髓瘤細(xì)胞
NCI-H929 人骨髓瘤細(xì)胞
CRL-5803 NCI-H1299
U266 人骨髓瘤細(xì)胞
EJ 人膀胱癌細(xì)胞
HOC 大鼠肝卵圓細(xì)胞
RWPE-1 人正常前列腺上皮細(xì)胞
CAL 27 人舌鱗癌細(xì)胞
Calu-3 人肺腺癌細(xì)胞
Eca-109 人食管癌細(xì)胞
DLD-1 人結(jié)直腸腺癌上皮細(xì)胞
5637.00 人膀胱癌細(xì)胞
SK-OV-3 人卵巢癌細(xì)胞
GBC-SD 人膽囊癌細(xì)胞
BEL7402 人肝癌細(xì)胞系
SW620 人結(jié)腸癌細(xì)胞
MRC-5 人胚肺成纖維細(xì)胞 細(xì)胞系購(gòu)買
PC9 人肺癌細(xì)胞
PC14 人肺癌細(xì)胞
BxPC 人原位胰腺腺癌細(xì)胞
PK（15） 豬腎上皮細(xì)胞
MDCK 狗腎上皮細(xì)胞
3D4/21 豬肺泡巨噬細(xì)胞
CRL-5803 NCI-H1299
MHCC-97L 人肝癌細(xì)胞（低轉(zhuǎn)移）
MHCC-97H 人肝癌細(xì)胞系（高轉(zhuǎn)移）
MiaCaPa-2 人胰腺癌細(xì)胞
HN13 口腔鱗狀細(xì)胞癌細(xì)胞
Bend3 小鼠腦微血管內(nèi)皮細(xì)胞
QBC939 人膽管癌細(xì)胞
Ishikawa 人子宮內(nèi)膜癌細(xì)胞
A498 人腎癌細(xì)胞
143B
WEHI-231 小鼠B淋巴細(xì)胞
BN
P9R 小鼠骨髓基質(zhì)細(xì)胞
P9 小鼠骨髓基質(zhì)細(xì)胞
F98 大鼠腦膠質(zhì)瘤細(xì)胞
NCIH1299
NCIH1650-M3
293LVT 人胚腎細(xì)胞--用于慢病毒包裝
EC9706 人食管癌細(xì)胞
CNE 人鼻咽癌細(xì)胞
H322
Panc O2
S8 BEL7402 人肝癌細(xì)胞系
R5 細(xì)胞系購(gòu)買
Pig MSC 豬骨髓間質(zhì)干細(xì)胞
HCAEC 人冠狀動(dòng)脈內(nèi)皮細(xì)胞
marc-145 猴胚胎腎上皮細(xì)胞
3D4/21 豬肺泡巨噬細(xì)胞
PK（15） 豬腎上皮細(xì)胞
MADB-106 大鼠乳腺癌細(xì)胞
Sf9 昆蟲(chóng)卵巢細(xì)胞
DADY
HUVEC[HUV-EC-C] 人臍靜脈內(nèi)皮細(xì)胞
PC14 人肺癌細(xì)胞
PC9 人肺癌細(xì)胞
Eca-109 人食管癌細(xì)胞
CAL 27 人舌鱗癌細(xì)胞
HOC 肝卵圓細(xì)胞
EJ 人膀胱癌細(xì)胞
QBC939 人膽管癌細(xì)胞
U266 人骨髓瘤細(xì)胞
293-mTLR5 人胚腎細(xì)胞
Raw-Blue 小鼠單核細(xì)胞白血病細(xì)胞
HL-7702 人正常肝細(xì)胞
Hep3B 人肝癌細(xì)胞
U-373MG 人腦神經(jīng)膠質(zhì)瘤細(xì)胞
Tca-8113 人舌鱗癌細(xì)胞
SNU-739 人肝癌細(xì)胞
SNU-638 人胃癌細(xì)胞
NUGC-3 人胃癌細(xì)胞
NIH-3T3 小鼠成纖維細(xì)胞
MKN-45 人胃癌細(xì)胞
LLC-PK1 豬腎近曲小管上皮細(xì)胞
Hs 746T 人胃癌細(xì)胞
Hepa1-6 小鼠肝癌細(xì)胞
HCT-8 人回盲腸癌細(xì)胞
BEL7402 人肝癌細(xì)胞系
HaCaT 人永生化表皮細(xì)胞
GES-1 人胃上皮細(xì)胞
B16-F0 小鼠黑色素瘤細(xì)胞
AZ-521 人胃癌細(xì)胞
786-O 人腎透明細(xì)胞腺癌細(xì)胞
208F 大鼠成纖維細(xì)胞
293TN 人胚腎細(xì)胞--用于慢病毒包裝
293FT 人胚腎細(xì)胞-用于慢病毒包裝
GP-293 人胚腎細(xì)胞
293-L.P. 人胚腎細(xì)胞
CRL-5803 NCI-H1299
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