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Merck蛋白抽提
MERCK蛋白抽提
1、BugBuster®蛋白抽提試劑——尤其適用于GST融合蛋白的抽提
BugBuster蛋白抽提試劑用于溫和破壞大腸桿菌細(xì)胞壁以釋放活性蛋白。作為激烈的機(jī)械法（如弗式壓榨法和超聲波法）的替代方法，這種試劑處理起來非常簡(jiǎn)便、快速，且成本低廉，適合制備表達(dá)蛋白用于下游的純化等應(yīng)用。
2、BugBuster Master Mix預(yù)混蛋白抽提試劑
BugBuster Master Mix是將BugBuster蛋白抽提試劑，Benzonase核酸酶和rLysozyme™溶菌酶溶液按*化配比混合好的一種非常方便使用的蛋白抽提試劑，有助于在*化條件下獲得可溶活性蛋白。
Product | Size | Cat.No. | Price（￥） |
BugBuster® Protein Extraction Reagent | 100 ml | 70584-3 | 750.00 |
500 ml | 70584-4 | 3,789.00 | |
BugBuster® Master Mix | 100 ml | 71456-3 | 1,700.00 |
500 ml | 71456-4 | 5,200.00 | |
CytoBuster™ Protein Extraction Reagent | 50 ml | 71009-3 | 824.00 |
250 ml | 71009-4 | 3,122.00 |
 | Comparison of E. coli lysis methods 50 ml samples of an induced 500 ml culture of BL21（DE3） containing pET-41a（+） encoding GST were harvested by centrifugation and resuspended in 2 ml 1X PBS, another commercially available protein extraction reagent, or BugBuster Reagent. The sample in PBS was sonicated with 10 pulses at 50% duty for 30 s total. Samples in lysis reagent were treated according to their respective protocols. Extracts were clarified by centrifugation and assayed for GST enzymatic activity. |
3、Benzonase® 核酸酶——賽多利斯病毒純化試劑盒推薦使用
應(yīng)用范圍
- ² 蛋白抽提操作中降低樣品粘度
- ² 雙向電泳中樣品制備過程
- ² 重組蛋白樣品制備中去除核酸污染
Product | Size | Cat.No. | Price（￥） |
Benzonase® Nuclease, Purity > 90% | 10 ku | 70746-3 | 1,200.00 |
2.5 ku | 70746-4 | 400.00 |
 | Viscosity reduction by Benzonase Nuclease E. coli BL21（DE3） cells containing a pET construct were suspended in BugBuster Reagent （5 ml/g wet weight）. Aliquots of the suspension were treated with the indicated amounts of Benzonase Nuclease for 10 min at |
