Invitrogen 18064-014 SuperScript? II Reverse Transcriptase 同類產品：

Invitrogen 18064-014 SuperScript? II Reverse Transcriptase 簡介：

產品名稱：SuperScript? II Reverse Transcriptase

分類：反轉錄酶

中文名稱：反轉錄酶II

品牌：Invitrogen

貨號：18064-014

規(guī)格：10,000 units

市場價格：3918元

特惠價格：致電

庫存狀態(tài)：現(xiàn)貨

Invitrogen 18064-014 SuperScript? II Reverse Transcriptase 英文描述：

SuperScript? II Reverse Transcriptase (RT) is an improved version of SuperScript? RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript? II RT superior performance characteristics.
? Yield – Reduced RNase H activity results in greater first-strand cDNA yields
? Size of cDNA – Generation of RT-PCR products up to 12 kb
? Thermostability – Full activity at 42°C
? Flexibility – Uses either total or poly(A)⁺ RNA
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of SuperScript? II is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)_12-18 as template?primer (3).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl₂, 1 mM DTT, 0.5 mM [³H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)_12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

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