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產(chǎn)品名稱：Invitrogen K494500 BLOCK IT U6 ENTRY VECTOR KIT
貨 號：K494500
規(guī) 格：20 RXNS
分 類：RNAi 載體
品 牌：Invitrogen
國產(chǎn)/進口：進 口
儲存溫度：-20°C
特惠價格：致 電
庫存狀態(tài)：現(xiàn) 貨
銷售商：上海慧穎生物科技有限公司
慧穎生物專業(yè)供應Invitrogen K494500 BLOCK IT U6 ENTRY VECTOR KIT，常備現(xiàn)貨，*。主營Life Invitrogen產(chǎn)品，囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規(guī)化學試劑、培養(yǎng)基及原料試劑、各類胎牛血清、馬血清等，如有需要，詳詢。
Invitrogen K494500 BLOCK IT U6 ENTRY VECTOR KIT描述：
The BLOCK-iT? U6 RNAi Entry Vector provides a simple, streamlined approach to cloning short hairpin RNA (shRNA) sequences for testing in transient transfections for RNA interference (RNAi), a preferred technique for analyzing gene downregulation. An easy cloning process places an ~50-bp oligonucleotide DNA immediay following a U6 pol III type promoter (Figure 1). Expression of this RNAi cassette forms a shRNA molecule in the cell that will be processed and act as short interfering RNA (siRNA) that will generate the RNAi effect.
Delivery of the U6 RNAi Cassette
Once cloning is complete, the U6 Entry Vector is ready to be used immediay in transient transfections using a reagent such as Lipofectamine? 2000. This makes this system ideal for initial shRNA screenings in many mammalian cell types. As an alternative in hard-to-transfect or non-dividing cell types, or to deliver to animal model systems, the RNAi cassette can easily be recombined into a BLOCK-iT? Viral RNAi vector. For stable shRNA lentiviral delivery and expression, recombine the BLOCK-iT? U6 Entry Vector with the pLenti6/BLOCK-iT? RNAi Vector. For transient delivery to challenging cell types using adenoviral transduction, recombine with the pAd/BLOCK-iT? RNAi Vector.
Invitrogen K494500 BLOCK IT U6 ENTRY VECTOR KIT同類產(chǎn)品主要有:
| 貨號 | 名稱 | 規(guī)格 |
| 18064-014 | SuperScript? II Reverse Transcriptase | 10,000U |
| 18080-044 | SuperScript? III Reverse Transcriptase | 10,000U |
| 18418-012 | Oligo(dT)12-18 Primer | 25ug |
| 18418-020 | Oligo(dT)20 Primer | 50ul(50uM) |
| 10777-019 | RNaseOUTTM Ribonuclease Inhibitor | 5,000U |
| 18080-051 | SuperScript? III First-Strand | 50 rxn |
| Synthesis System for RT-PCR | ||
| 11668-027 | Lipofectamine TM 2000 Reagent | 0.75ml |
| 11668-019 | 1.5ml | |
| 13778-150 | Lipofectamine RNAiMax | 1.5ml |
| 13778-075 | Lipofectamine RNAiMax | 0.75ml |
| 15596-026 | TRlzol ?Reagent | 100ml |
| 15596-018 | TRlzol ?Reagent | 200ml |
| 10296028 | TRIZOL LS Reagent 200 ml（長方形） | 200ml |
| 10296010 | TRIZOL LS Reagent 100ml（長方形） | 100ml |
| 11789020 | BP CLONASE II ENZYME MIX | 20 REACTIONS |
| 11791020 | LR CLONASE II ENZYME MIX | 20 REACTIONS |
| 12328040 | AMV | 100 REACTIONS |
| (CLON AMV FIRST STR SYNTH KIT) | ||
| 28025-013 | M-MLV Reverse Transcriptase | 40,000U |
| 17504-044 | B-27（黃顏色） | 10ml |
| 12587-010 | B-27（黃顏色）不含維生素A | 10ml |
| 15090-046 | 2.5% Trypsin (10X), no Phenol Red | 100ml |
| 15050-065 | 0.25% Trypsin(1X),Phenol Red | 100ml |
| 15050-057 | 500ml | |
| 15140-122 | Pen Strep | 100ml |
| 15140-163 | 20X100ml | |
| 25200-056 | 0.25% Trypsin-EDTA(1X,Phenol Red) | 100ml |
| 25200-114 | 20X100ml | |
| 25300-054 | 0.05% Trypsin(1X),Phenol Red: | 100ml |
| 25300-120 | 20X100ml | |
| 15400-054 | 0.5% Trypsin(10X),: | 100ml |
| 25030-081 | L-Glutamine 200mM | 100ml |
| 25030-164 | 20X100ml | |
| 12328019 | CLONED AMV RT, 750U | TRANSCRIPTASE |
| 12328032 | CLONED AMV FIRST STRA SYN KIT | 25 REACTIONS |
| 10966-018 | Platinum? Taq DNA聚合酶 | 100 rxns |
| 10966-026 | 250 rxns | |
| 10966-034 | 500 rxns | |
| K210007 | PURELINK PLASMID MAXI 25 | 25 REACTIONS |
| 15224041 | T4 DNA LIGASE HI CONC. | 250 U |
| C737303 | ONE SHOT STBL3 COMP E COLI | ONE SHOT STBL3 20 RXN |
| 12033015 | ELECTROMAX DH10B T1 CELLS | 0.5 ML (5 X 0.1 ML) |
| A10460 | ONE SHOT CCDB SURVIVAL 2-T1R | 10 REACTIONS |
| LC5925 | SEEBLUE PLUS2 | 500UL |
| 11708039 | PLATINUM PFX DNA POLYMERASE | 500 REACTIONS #95514- #52806 |
| 12344024 | ACCUPRIME PFX DNA POLYMERASE | 200 REACTIONS |
| 12346086 | ACCUPRIME TAQ HIFI | 200 RXNS |
| 11495017 | PCRX ENHANCER SYSTEM | 250 RXN 52395 |
| 450008 | TOPO XL PCR CLONING KIT | 10 RXN |
| MECOV50 | METHYLCODE BISULFITE CONV KIT | 50 REACTIONS |
| 12935300 | STEALTH RNAI NEG CTL MED GC | 250 μl |
| 2013 | FLUORESCENT OLIGOMER, 20UM | 250 UNITS |
| K159303 | KIT,FAST TRACK | 18 RXN |
| 11917020 | SS DBL STRND CDNA SYN KIT | 50 RXNS |
| LC5677 | MARK12 W RANGE MW STD,1ML | 1 ML |
Invitrogen產(chǎn)品種類較多，本展臺無法一一體現(xiàn)，以上是部分產(chǎn)品，若有需要的產(chǎn)品，請及時與我們?nèi)〉茫?/span>咨詢。
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