Recombinant UDP Glucose Ceramide Glucosyltransferase (UGCG) 

UDP葡萄糖神經(jīng)酰胺葡萄糖基轉(zhuǎn)移酶(UGCG)重組蛋白

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues:  Lys39~Leu171

Tags: N-terminal His Tag

Tissue Specificity: Testis.

Subcellular Location: Golgi apparatus membrane; Multi-pass membraneprotein.

Purity: > 95%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.7

Predicted Molecular Mass:  16.1kDa

Accurate Molecular Mass:  16kDa as determined by SDS-PAGE reducingconditions.

Note: 98% cross-reactivity of UGCG was observed among human, mouseandrat.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

            Store at 2-8oC for one month.

            Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

[  IDENTIFICATION  ]

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