InvivoGen 新品推薦：核酸轉(zhuǎn)染增強(qiáng)試劑——NATE™

產(chǎn)品介紹：

NATE™是InvivoGen設(shè)計(jì)的一種核酸轉(zhuǎn)染增強(qiáng)劑，可以提高難轉(zhuǎn)染細(xì)胞的瞬轉(zhuǎn)與穩(wěn)轉(zhuǎn)效率，尤其適合人的單核細(xì)胞與小鼠巨噬細(xì)胞等難轉(zhuǎn)染的細(xì)胞，如外周血單核細(xì)胞（THP-1）和小鼠單核巨噬細(xì)胞白血病細(xì)胞（RAW 264.7），且只需在平時(shí)加轉(zhuǎn)染試劑操作前30min加入NATE™即可。值得注意的是，NATE對(duì)細(xì)胞溫和，不會(huì)對(duì)細(xì)胞培養(yǎng)產(chǎn)生任何進(jìn)一步的毒性。

在真核細(xì)胞轉(zhuǎn)染過程中，外源性核酸(如質(zhì)粒)的主要障礙是會(huì)被胞質(zhì)傳感器檢測，如cGAS/STING、AIM2炎性小體和LC3介導(dǎo)的自噬。這些防御信號(hào)級(jí)聯(lián)的激活常常會(huì)降低轉(zhuǎn)染率和細(xì)胞存活率，特別是在難以轉(zhuǎn)染的細(xì)胞(如免疫細(xì)胞)中會(huì)更明顯。當(dāng)使用NATE™時(shí)，這些核酸傳感通路將被抑制，從而在轉(zhuǎn)染過程中保護(hù)質(zhì)粒并促進(jìn)其表達(dá)。

產(chǎn)品特色：

● 與常用轉(zhuǎn)染試劑 (e.g. GeneXPlus, Lipofectamine® LTX, and jetPRIME®) 及物理方法兼容。

● 更高的轉(zhuǎn)染率，也適用于大質(zhì)粒 (> 10kb)。

● 在所有的轉(zhuǎn)染測試方案中都顯示對(duì)細(xì)胞溫和，沒有毒性。

產(chǎn)品信息：

應(yīng)用舉例：

Top - Transient transfection of an ~3 kb GFP-expressing plasmid into THP-1 cells was performed using GeneXPlus both in the absence (left) and presence (right) of NATE™. After 48 hours, the transfected cells expressing GFP were visualized by fluorescence microscopy. Bottom - Transient transfections of an ~3 kb GFP-expressing plasmid (left) and an 8 kb GFP-expressing plasmid (right) into THP-1 cells was performed using commonly used transfection methods such as GeneXPlus, Lipofectamine® LTX, jetPRIME®, and nucleofection. This was performed both in the presence (green) and absence (yellow) of NATE™. After 48 hours, transfection efficiency (% GFP-expressing cells) was measured using flow cytometry. Data are presented as a fold change compared to transfection without NATE™ 

Top - Transient transfection of an ~3 kb GFP-expressing plasmid into RAW 264.7 cells was performed using Lipofectamine® LTX both in the absence (left) and presence (right) of NATE™. After 48 hours, the transfected cells expressing GFP were visualized by fluorescence microscopy. Bottom - Transient transfection of an ~3 kb GFP-expressing plasmid (left) and an 8 kb GFP-expressing plasmid (right) into RAW 264.7 cells was performed using commonly used transfection methods including Lipofectamine® LTX, jetPRIME®, FuGENE®, and nucleofection. This was performed both in the presence (green) and absence (yellow) of NATE™. After 48 hours, transfection efficiency (% GFP-expressing cells) was measured using flow cytometry. Data are presented as fold change compared to transfection without NATE™.

Stable transfection of an ~10 kb SEAP?expressing plasmid into RAW 264.7 cells was performed using Lipofectamine® LTX both in the absence (left) and presence (right) of NATE™. After 10 days in selection with Blasticidin, the number of stable clones expressing SEAP (blue wells) was visualized using QUANTI?Blue™, a SEAP detection reagent.

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