細(xì)胞名稱

人卵巢癌細(xì)胞PEO-1

貨物編碼

BFN60808418

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級(jí)

1

用途限制

僅供科研        1類

培養(yǎng)體系

DMEM高糖+10%FBS+1%三抗

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡介

人卵巢癌細(xì)胞PEO-1取自女性供體，年齡不詳。貼壁培養(yǎng)，不規(guī)則形態(tài)。

注釋

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

Part of: OCCP ovarian cancer cell line panel.

Doubling time: 53 hours (PubMed=3583449); 37 hours (PubMed=3167863); 37.48 hours 

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

基因突變

Hemizygous for BRCA2 p.Tyr1655Ter (5193C>G) (PubMed=19654294; PubMed=25230021).

TP53 p.Gly244Asp (c.731G>A) (PubMed=25230021).

HLA信息

Class I

HLA-A        A*03:01,03:01

HLA-B        B*07:02,07:02

HLA-C        C*07:02,07:02

Class II

HLA-DQ        DQB1*03:04,03:04

STR信息

Amelogenin        X

CSF1PO        10,12

D2S1338        20,21

D3S1358        16

D5S818        11,12

D7S820        10

D8S1179        13,14 (PubMed=22710073; PubMed=25230021)

13 (PubMed=25877200)

D13S317        10

D16S539        9

D18S51        16,17

D19S433        13,15

D21S11        32.2

FGA        20

Penta D        14

Penta E        11,12

TH01        9.3

TPOX        9,11

vWA        15,16

參考文獻(xiàn)

PubMed=8612712; DOI=10.1006/excr.1996.0145

Perreault N., Beaulieu J.-F.

Use of the dissociating enzyme thermolysin to generate viable human normal intestinal epithelial cell cultures.

Exp. Cell Res. 224:354-364(1996)

PubMed=9827699; DOI=10.1002/(SICI)1097-4644(19981215)71:4<536::AID-JCB8>3.0.CO;2-1

Desloges N., Basora N., Perreault N., Bouatrouss Y., Sheppard D., Beaulieu J.-F.

Regulated expression of the integrin alpha9beta1 in the epithelium of the developing human gut and in intestinal cell lines: relation with cell proliferation.

J. Cell. Biochem. 71:536-545(1998)

PubMed=20444515; DOI=10.1016/j.ijfoodmicro.2010.03.026

Cencic A., Langerholc T.

Functional cell models of the gut and their applications in food microbiology -- a review.

Int. J. Food Microbiol. 141:S4-14(2010)

PubMed=28239090; DOI=10.3390/foods1010040

Trapecar M., Cencic A.

Application of gut cell models for toxicological and bioactivity studies of functional and novel foods.

Foods 1:40-51(2012)