細胞名稱

人食管上皮細胞HET-1A

貨物編碼

BFN60806666

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運輸方式

常溫保溫運輸

干冰運輸

安全等級

1

用途限制

僅供科研           1類

培養(yǎng)體系

DMEM高糖+10%FBS+1%雙抗

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡介

人食管上皮細胞HET-1A取自25歲男性。

序列突變

/

注釋

Doubling time: 27.4 +- 3.6 hours (PubMed=21127259).

Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [pRSV-T].

Omics: Deep RNAseq analysis.

Omics: Transcriptome analysis.

STR信息

Amelogenin        X,Y

CSF1PO        10,12

D5S818        11,12

D7S820        9

D13S317        11

D16S539        9,11

TH01        7

TPOX        11

vWA        16

參考文獻

Patent=WO1991005062A1

Stoner G.D., Reddel R.R., Harris C.C.

Human esophageal epithelial cell lines.

Patent number WO1991005062A1, 18-Apr-1991

PubMed=1703038

Stoner G.D., Kaighn M.E., Reddel R.R., Resau J.H., Bowman D., Naito Z., Matsukura N., You M., Galati A.J., Harris C.C.

Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells.

Cancer Res. 51:365-371(1991)

PubMed=8504475; DOI=10.1093/carcin/14.5.833

Lehman T.A., Modali R., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C.

p53 mutations in human immortalized epithelial cell lines.

Carcinogenesis 14:833-839(1993)

PubMed=20139130; DOI=10.1093/carcin/bgq036

Duggan S.P., Behan F.M., Kirca M., Smith S.M., Reynolds J.V., Long A., Kelleher D.P.

An integrative genomic approach in oesophageal cells identifies TRB3 as a bile acid responsive gene, downregulated in Barrett's oesophagus, which regulates NF-kappaB activation and cytokine levels.

Carcinogenesis 31:936-945(2010)

PubMed=20843300; DOI=10.1042/BC20100071

Underwood T.J., Derouet M.F., White M.J., Noble F., Moutasim K.A., Smith E., Drew P.A., Thomas G.J., Primrose J.N., Blaydes J.P.

A comparison of primary oesophageal squamous epithelial cells with HET-1A in organotypic culture.

Biol. Cell 102:635-644(2010)

PubMed=21127259; DOI=10.1152/ajpgi.00461.2010

Goldman A., Chen H.D.R., Roesly H.B., Hill K.A., Tome M.E., Dvorak B., Bernstein H., Dvorak K.

Characterization of squamous esophageal cells resistant to bile acids at acidic pH: implication for Barrett's esophagus pathogenesis.

Am. J. Physiol. Gastrointest. Liver Physiol. 300:G292-G302(2011)

PubMed=28224081; DOI=10.1016/j.gdata.2017.02.002

Akagi I., Ishibashi O.

RNA-Seq analysis of human cell lines established from normal and neoplastic esophageal squamous epithelium.

Genomics Data 12:4-6(2017)