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| 供貨周期 | 一個月 |
|---|
產(chǎn)品簡介
詳細(xì)介紹
產(chǎn)品詳情：
Cube Biotech公司的GST/Gluthathione親和瓊脂糖已成功發(fā)表在以下出版物：
PureCube Glutathione磁珠的效率:
圖1：可靠的批次間再現(xiàn)性。GST從大腸桿菌裂解物中純化，利用三個獨立生產(chǎn)不同批次的PureCube Glutathione磁珠。FT：穿流，E1-E5：洗脫組分。我們的GST標(biāo)簽純化產(chǎn)品能夠復(fù)制所有的純化試驗從批次到批次。產(chǎn)品在多次使用后實現(xiàn)了盡可能小的波動，并且多次連續(xù)的GST標(biāo)簽純化在使用相同的磁珠的情況下能確保相同的高質(zhì)量。
產(chǎn)品參數(shù)：
應(yīng)用 | Specific binding and purification of GST-tagged proteins |
特異性 | Affinity to GST-tagged proteins |
結(jié)合能力 | 10 mg / ml |
Bead Ligand | Protein purification beads with GST-Antibody |
粒徑大小 | 40 μm |
pH穩(wěn)定性 | 3-12 |
推薦流速 | 0.25-1.0 mL/min |
推薦操作壓力 | <5bar (72 psi) |
保存溫度 | 2℃到8℃ |
訂購詳情：
貨號 | 產(chǎn)品 | 粒徑 （μm） | 每毫升磁 珠載量 | 運(yùn)輸 溫度 | 儲存 溫度 | 應(yīng)用 |
32101 | PureCube Glutathione Agarose(1mL) | 40 | 10mg/mL | 室溫 | 4° C | 用于純化含GST的重組融合蛋白或依賴轉(zhuǎn) S- 移酶或Glutathione的蛋白 ,pH 穩(wěn)定工作范圍3-12, 最大流速為6mL/min |
32103 | PureCube Glutathione Agarose(10mL) | 40 | 10mg/mL | 室溫 | 4° C | |
32105 | PureCube Glutathione Agarose(50mL) | 40 | 10mg/mL | 室溫 | 4° C | |
32110 | PureCube Glutathione Agarose(250mL) | 40 | 10mg/mL | 室溫 | 4° C | |
32112 | PureCube Glutathione Agarose(500mL) | 40 | 10mg/mL | 室溫 | 4° C | |
32115 | PureCube Glutathione Agarose(1000mL) | 40 | 10mg/mL | 室溫 | 4° C | |
32120 | PureCube Glutathione Agarose(5000mL) | 40 | 10mg/mL | 室溫 | 4° C |
文獻(xiàn)參考：
1.Kilkenny, M. L et al. “The human CTF4-orthologue AND-1 interacts with DNA polymerase α/primase via its unique C-terminal HMG box." Open biology vol. 7,11 (2017): 170217. doi:10.1098/rsob.170217.
2. Napolitano, Anna et al. “Cysteine-Reactive Free ISG15 Generates IL-1β-Producing CD8α+ Dendritic Cells at the Site of Infection." Journal of immunology (Baltimore, Md. : 1950) vol. 201,2 (2018): 604-614. doi:10.4049/jimmunol.1701322
3. Boor, Susanne de (2015). Mechanistic studies on the regulation of Ran-function by post-translational lysine-acetylation. Dissertation, Universit?t zu K?ln.
4. Fischer K. (2015). TBX3-Mutationsanalysen und Konstruktion einer Zelllinie für TBX2-Inhibitorscreenings. Dissertation, Universit?t zu Mainz.
5. A. Sommerkamp, et al. (2019). Crystal structure of the first eukaryotic bilin reductase Gt PEBB reveals a flipped binding mode of dihydrobiliverdin. Journal of Biological Chemistry. jbc.RA119.009306. 10.1074/jbc.RA119.009306.
6. Chiocchini C., Trefzer A., Vattem K., Kuhn P., inventor; Thermo Fisher Scientific Geneart GmBH, Pierce Biotechnology Inc, assignee. Devices and methods for producing nucleic acids and proteins. United States patent US20180291413A1. 2016 April 10.
7. Chiocchini, C. et al. (2020). From Electronic Sequence to Purified Protein Using Automated Gene Synthesis and In Vitro Transcription/Translation. ACS Synthetic Biology. XXXX. 10.1021/acssynbio.0c00060.