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        1. 北京云肽生物科技有限公司
          中級(jí)會(huì)員 | 第1年

          13269198101

          生化試劑
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          ARBOR ASSAYS 免疫測(cè)試產(chǎn)品 試劑盒

          時(shí)間:2024/8/1閱讀:148
          分享:

          ARBOR ASSAYS 免疫測(cè)試產(chǎn)品 試劑盒

           

          Arbor Assays公司成立于2007年,位于密歇根州的安娜堡,公司的三位主創(chuàng)始人均為有多年科研經(jīng)驗(yàn)的生命和臨床科學(xué)家。其宗旨是為臨床使用的重要生物分子建立高質(zhì)量的檢測(cè)和免疫測(cè)試產(chǎn)品。在生物領(lǐng)域建立健全的免疫檢測(cè)和酶活性試劑盒以定量生物分子。

          主要為臨床重要生物分子提供高質(zhì)量的檢測(cè)和免疫測(cè)試產(chǎn)品,如乙酰膽堿酯酶活性熒光檢測(cè)試劑盒、谷胱甘肽熒光檢測(cè)試劑盒、血紅蛋白比色檢測(cè)試劑盒等。種類(lèi)多樣,操作簡(jiǎn)單,靈敏度高。

          認(rèn)識(shí)到對(duì)靈敏度、速度、便利性、質(zhì)量和服務(wù)的需求,我們研究、開(kāi)發(fā)和制造用于臨床重要生物分子的最高質(zhì)量的檢測(cè)和免疫測(cè)定試劑盒。

          Arbor Assays公司的主要產(chǎn)品包括:

          DetectX® Kits

          Inhibitors & Activators

          AbX® Antibodies & Affinity Resins

          Detection Systems

          Components & Ancillary Reagents

          所需材料:

          類(lèi)固醇標(biāo)準(zhǔn)品,用于檢測(cè)萃取效率

          ACS級(jí)乙醇(或乙酸乙酯)

          玻璃試管

          處理流程:

          確保樣品wan全干燥,并對(duì)樣品進(jìn)行粉末化處理,以提高萃取回收率。如果可能的話清除任何大顆粒,如草屑。我們建議通過(guò)使用試劑盒的AB分析緩沖液及已知濃度的類(lèi)固醇溶液檢測(cè)結(jié)果來(lái)確定提取效率,詳細(xì)信息請(qǐng)參考下面的萃取效率部分。乙醇或乙酸乙酯萃取樣品及萃取效率控制如下:

          1.稱(chēng)取≥0.2 g干燥的糞便固體放入管中。樣品可以通過(guò)被動(dòng)干燥、溫和加熱(≤60°C)或冷凍的方式來(lái)進(jìn)行干燥處理。

          2.每0.1 g固體(0.1 g糞便固體/mL)加入1 mL乙醇(或乙酸乙酯)并密封。

          3.用力搖晃至少30分鐘。

          4.在4°C下,將樣品在5,000 rpm下離心15分鐘。取上清放入一個(gè)干凈的管中。如果密封得當(dāng),該樣本可在≤-20°C下保存至少一個(gè)月。

          注:含有低水平分析物的樣品可以通過(guò)干燥提取液和在少量分析緩沖液中重懸,來(lái)達(dá)到濃縮的效果。用乙酸乙酯提取的樣品在加入緩沖液之前,必須wan全干燥以去除溶劑。從步驟4中轉(zhuǎn)移測(cè)量體積(蒸發(fā)體積)的上清到一個(gè)干凈的管中,在離心蒸發(fā)裝置或氮?dú)庀抡舭l(fā)至干燥。干燥、提取的樣品可在干燥器中冷凍≤-20°C保存?;蛴靡掖?如100μL)溶解干燥提取的樣品,直接進(jìn)行步驟5。

           5.以上步驟為通用步驟,可根據(jù)需要量進(jìn)行等比例體積調(diào)整。將步驟4中提取的樣本上清液100μL加入最少400μL的AB緩沖液中(總重構(gòu)體積=100μL+400μL)。室溫下渦旋混勻,靜置5min,重復(fù)至少兩次渦旋和靜置步驟。對(duì)于免疫分析,測(cè)定中乙醇含量通常需要≤5%,需要根據(jù)所選擇的試劑盒中描述的最大乙醇含量耐受情況,用檢測(cè)緩沖液進(jìn)行稀釋。

          6.根據(jù)試劑盒說(shuō)明書(shū)對(duì)稀釋后的樣本進(jìn)行ELISA檢測(cè)。

           

          下面是ARBOR ASSAYS公司的部分熱銷(xiāo)產(chǎn)品

          產(chǎn)品名稱(chēng)

          貨號(hào)

          規(guī)格

          Goat Anti-Mouse IgG Fc, 25MG

          A008-25MG

          25MG

          Cyclic AMP Direct ELISA Kit (1 Plate)

          K019-H1

          1x96 well plate

          Corticosterone ELISA Kit (1 Plate)

          K014-H1

          1x96 well plate

          Protein Kinase A (PKA) Activity Assay Kit (1 Plate)

          K027-H1

          1x96 well plate

          Aldosterone ELISA Kit (1 Plate)

          K052-H1

          1x96 well plate

          Urea Nitrogen (BUN) Detection Kit (2 Plate)

          K024-H1

          2x96 well plate

          Cortisol ELISA Kit (1 Strip plate)

          K003-H1

          1x96 well plate

          PGFM ELISA Kit (1 Plate)

          K022-H1

          1x96 well plate

          Triiodothyronine (T3) ELISA Kit (1 Plate)

          K056-H1

          1 Kit

          FRAP™ (Ferric Reducing Antioxidant Power) Detection Kit (2 Plate)

          K043-H1

          2x96 well plate

          Hemoglobin Detection Reagent, 20ML

          C038-20ML

          20ML

          Ceruloplasmin Colorimetric Activity Kit (2 Plate)

          K035-H1

          2x96 well plate

          ThioStar™ Fluorescent Thiol Substrate, 100UG

          L002-100UG

          100UG

          Cyclic GMP Direct Chemiluminescent ELISA Kit (1 Plate)

          K020-C1

          1x96 well plate

          Progesterone ELISA Kit (1 Plate)

          K025-H1

          1x96 well plate

          Testosterone ELISA Kit (1 Plate)

          K032-H1

          1x96 well plate

          Estrone-3-Glucuronide (E1G) ELISA Kit (1 Plate)

          K036-H1

          1x96 well plate

          Thyroxine (T4) ELISA Kit (1 Plate)

          K050-H1

          1x96 well plate

          Prostaglandin E2 (PGE2) Multi-Format ELISA Kit (1 Plate)

          K051-H1

          1x96 well plate

          Tranylcypromine

          X042-1EA

          1EA

          Triiodothyronine (T3) ELISA Kit (5 Plate)

          K056-H5

          5x96 well plate

          Glutathione Colorimetric Detection Kit (4 Plate)

          K006-H1

          4x96 well plate

          Cyclic GMP Direct ELISA Kit (1 Plate)

          K020-H1

          1x96 well plate

          Progesterone ELISA Kit (5 Plate)

          K025-H5

          5x96 well plate

          Cyclic AMP Direct ELISA Kit (5 Plate)

          K019-H5

          5x96 well plate

          Estradiol ELISA Kit (1 Plate)

          K030-H1

          1x96 well plate

          Oxytocin ELISA Kit (1 Plate)

          K048-H1

          1x96 well plate

          Estrone-3-Glucuronide (E1G) ELISA Kit (5 Plate)

          K036-H5

          5x96 well plate

          Pregnanediol-3-Glucuronide (PDG) ELISA Kit (5 Plate)

          K037-H5

          5x96 well plate

          Hemoglobin Standard, 300UL

          C037-300UL

          300UL

          DetectX® Estrone-3-Sulfate (E1S) Antibody, 3ML

          C133-3ML

          3ML

          Creatinine Urinary Detection Kit (2 Plate)

          K002-H1

          2x96 well plate

          Butyrylcholinesterase Fluorescent Activity Kit (2 Plate)

          K016-F1

          2x96 well plate

          Corticosterone ELISA Kit, (5 Plate)

          K014-H5

          5x96 well plate

          Cyclic GMP Direct Chemiluminescent ELISA Kit (5 Plate)

          K020-C5

          5x96 well plate

          Superoxide Dismutase (SOD) Colorimetric Activity Kit (2 Plate)

          K028-H1

          2x96 well plate

          Catalase Colorimetric Activity Kit (2 Plate)

          K033-H1

          2x96 well plate

          Glutathione Fluorescent Detection Kit (1 Plate)

          K006-F1

          1x96 well plate

          Pregnanediol-3-Glucuronide (PDG) Standard, 625UL

          C128-625UL

          625UL

          Thyroxine (T4) ELISA Kit (5 Plate)

          K050-H5

          5x96 well plate

          Acetylcholinesterase Fluorescent Activity Kit (2 Plate)

          K015-F1

          2x96 well plate

          Creatinine Serum Kit (2 Plate)

          KB02-H1

          2x96 well plate

          Hydrogen Peroxide Fluorescent Detection Kit (2 Plate)

          K034-F1

          2x96 well plate

          Creatinine Serum Low Sample Volume Kit (384-well Plate)

          KB02-H1D

          1x384 well plate

          Recombinant Obelin, 100UG

          L001-100UG

          100UG

          Dry DMSO, 20ML

          X022-20ML

          20ML

          Dry DMSO, 2ML

          X022-2ML

          2ML

          Urea Nitrogen Standard, 1ML

          C089-1ML

          1ML

          NADPH lyophilized, 1EA

          X045-1EA

          1EA

          DetectX® PGE2 Conjugate, 13ML

          C179-13ML

          13ML

          17-Hydroxyprogesterone ELISA Kits

          K053-H

          1 Kit

          Arg8-Vasopressin (AVP) ELISA Kits

          K049-H

          1 Kit

          Arg8-Vasopressin (AVP) Chemiluminescent ELISA Kits

          K049-C

          1 Kit

          Androstenedione ELISA Kits

          K070-H

          1 Kit

          Aldosterone Chemiluminescent ELISA Kits

          K052-C

          1 Kit

          Allopregnanolone ELISA Kits

          K061-H

          1 Kit

          Adrenocorticotropic Hormone (ACTH) ELISA Kits

          K072-H

          1 Kit

          Alkaline Phosphatase (ALP) Colorimetric Activity Kit

          K082-H1

          1 Kit

          3′,3′-Cyclic GAMP ELISA Kits

          K073-H

          1 Kit

          20-Hydroxyecdysone ELISA Kit

          K066-H

          1 Kit

          2′,3′-Cyclic GAMP STING-Based FRET Detection Kits

          K081-F

          1 Kit

          2′,3′-Cyclic GAMP ELISA Kits

          K067-H

          1 Kit

          Cyclic GMP Direct ELISA Kits – Improved Sensitivity

          K065-H

          1 Kit

          Endothelin-1 (ET-1) ELISA Kit

          K045-H1

          1 Kit

          Estrone-3-Sulfate (E1S) ELISA Kits

          K038-H

          1 Kit

          Assay Buffer, 5X

          X065-110ml

          110ml

          Blocking Buffer, 10X

          X109-25ml

          25ml

          Goat anti-Rabbit IgG Coated Plate

          X016-1EA


          HRP Conjugate Diluent

          X076-300ml

          300ml

          TMB Substrate

          X019-55ml

          55ml

          Mesotocin Solution (500ng/mL)

          X127-625UL


          5-Azacytidine

          P012-50MG

          50mg

           


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