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        1. 上海乾思生物科技有限公司
          中級(jí)會(huì)員 | 第16年

          18916423352

          細(xì)胞庫(kù)
          優(yōu)勢(shì)品牌
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          自產(chǎn)ELISA試劑盒,試劑盒
          進(jìn)口ELISA試劑盒
          人細(xì)胞,人細(xì)胞系,人細(xì)胞株
          3h biomedical,3H Biomedical AB,3hbiomedical,3-M Company,4adi,4rtilab,5 prime,5prime a & g pharmaceuticals,A&D Weighing,Aalto Scientific,Aalto Scientific ltd,Aalto Scientific,ltd.,aaltobioreagents,aaltoscientific,aat bioquest AAT Bioquest Inc,AB Biolabs,ab peptides,AB Sciex,Abaxis,Abazyme,Abberior,abbexa abbiotec,Abbkine,abbomax,Abbott Molecular,abcam,ABclonal,ABclonal Inc.,abcore-inc abcr GmbH,abcrGmbH,abcysonline,ABD,ABD Bioquest,Inc,abd serotec,ab-direct,abdserotec Abeomics,abfrontier,abgab,abgene,abgent,ABI Applied Biosystems,abiocode,abionline Abkem Iberia,ABL,Inc,Abliance,abm Inc.,abmart,abmgood,Abmole,Abmole Bioscience Inc. abnova,Abraxis,Absolute Antibody,ABT Beads,abt beads technologies,ABTbeads,abvision,Abways abzoom,academy biomed,academy bio-medical,acanthus research,acanthusresearch,ACC,accelagen,Accelagen inc. Access Biologicals,LLC,accessbiologicals,acciusa,accu-chek,AccuDiag Allergy,AccuDiag Anemia,AccuDiag Autoimmune Diseases,AccuDiag Bone Metabolism AccuDiag Thyroid,accumax accutase,Accumetrics,Accurate,accurate chemical,Accurate Wirecraft Company,accuratechemical,accustandard acdbio,Ace Glass,Ace Hose and Rubber Co,ACEA Biosciences,ACL Incorporated,ACME Microspheres Inc,ACMEmicrospheres,acris acris antibodies,Acris Antibodies GmbH,acris-antibodies,ACROBiosystems,acrometrix,acros,Acros Organics,ACT Test Panels LLC actc,actgene,active motif,activemotif,Adam Equipment,Inc,AddexBio,Addexbio Technologies,addgene ademtech,adg,ADI,ADInstruments,adipogen,Adolf Kuhner,adooq,Advance Tabco advanced immunochemical,advanced immunochemical inc,advanced targeting systems,Advansta,Advansta Inc.,Advansta,Inc,Advantech,Advent Electric Inc adventbio,advnt,advtechind,advy chemical,advy chemical Purified Proteins,advychemical Polyclonal Antisera,aesku,aetltd affiland,affinity,affinity biologicals,affinity bioreagent,Affinity BioReagents,Affinity BioReagents abr,Affinity Biosciences,affinitybiologicals AffinityBioReagents,AffinityImmuno,affix scientific,affixscientific,affymetrix,ag scientific,Agar Scientific,agarscientific agdia,Agela Technologies,agilent,Agilent Seahorse,agilent technologies,agilet,agilet solutions,agiletsolutions agrenvec,agrisera,AGRO-BIO,agscientific,ahdiagnostics,ahf,AHF analysentechnik,AHF analysentechnik AG Air Technology Solutions,Airgas Safety,Air-Tite,AJAX SCIENTIFIC LTD,AK Limited,Akro-Mils,ALA Scientfic,alamarblue Alba Bioscience,Alconox Inc,aldevron,alerchek,alere,Alexis,ALEXIS Biochemicals,Alfa Aesar Aline Biosciences,AlineBiosciences,All Foils Incorporated,Allcells,allele biotech,allelebiotech,allen-bradley,AllerMAbs
          大鼠細(xì)胞,大鼠細(xì)胞系
          細(xì)胞株,細(xì)胞系,傳代細(xì)胞
          白鰱細(xì)胞 斑馬魚(yú)細(xì)胞 豹貓細(xì)胞 成人細(xì)胞 赤狐細(xì)胞 大額牛細(xì)胞 大耳山羊細(xì)胞 大黃魚(yú)細(xì)胞 大長(zhǎng)舌果蝠細(xì)胞 俄勒岡地鼠細(xì)胞 非洲綠猴細(xì)胞 高背鯽魚(yú)細(xì)胞 果蠅細(xì)胞 果子貍細(xì)胞 荷蘭黑白花奶牛細(xì)胞 黑化大家鼠細(xì)胞 花鼠細(xì)胞 家兔細(xì)胞 昆蟲(chóng)細(xì)胞 綠猴細(xì)胞 馬鐵菊頭蝠細(xì)胞 綿羊細(xì)胞 三葉小蹄蝠細(xì)胞 散鱗鏡鯉細(xì)胞 社鼠細(xì)胞 鼠細(xì)胞 樹(shù)鼩細(xì)胞 團(tuán)頭魴細(xì)胞 新生兒細(xì)胞 新生牛細(xì)胞 敘利亞倉(cāng)鼠細(xì)胞 長(zhǎng)尾綠猴細(xì)胞 中國(guó)倉(cāng)鼠細(xì)胞 中華鱉細(xì)胞 棕果蝠細(xì)胞 腫瘤細(xì)胞 豬細(xì)胞 兔細(xì)胞 犬細(xì)胞
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          SIGMA現(xiàn)貨,SIGMA代理

          簡(jiǎn)述Elisa試驗(yàn)與操作使用

          時(shí)間:2011/9/26閱讀:1302
          分享:

               Elisa試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法。由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中。ELISA檢測(cè)試劑盒是用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎(HEV)病毒 IgM 抗體ELISA檢測(cè)。

                ELISA的基礎(chǔ)是抗原或抗體的固相化及抗原或抗體的酶標(biāo)記。結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學(xué)活性,酶標(biāo)記的抗原或抗體既保留其免疫學(xué)活性,又保留酶的活性。在測(cè)定時(shí),受檢標(biāo)本(測(cè)定其中的抗體或抗原)與固相載體表面的抗原或抗體起反應(yīng)。用洗滌的方法使固相載體上形成的抗原抗體復(fù)合物與液體中的其他物質(zhì)分開(kāi)。再加入酶標(biāo)記的抗原或抗體,也通過(guò)反應(yīng)而結(jié)合在固相載體上。此時(shí)固相上的酶量與標(biāo)本中受檢物質(zhì)的量呈一定的比例。加入酶反應(yīng)的底物后,底物被酶催化成為有色產(chǎn)物,產(chǎn)物的量與標(biāo)本中受檢物質(zhì)的量直接相關(guān),故可根據(jù)呈色的深淺進(jìn)行定性或定量分析。由于酶的催化效率很高,間接地放大了免疫反應(yīng)的結(jié)果,使測(cè)定方法達(dá)到很高的敏感度。 ELISA可用于測(cè)定抗原,也可用于測(cè)定抗體。  
          然而,影響Elisa試驗(yàn)結(jié)果的因素很多,故加強(qiáng)各個(gè)環(huán)節(jié)的質(zhì)量保證才能充分發(fā)揮其方法學(xué)的優(yōu)點(diǎn)。
           

          操作注意事項(xiàng):
          1. 試劑應(yīng)按標(biāo)簽說(shuō)明書(shū)儲(chǔ)存,使用前恢復(fù)到室溫。稀稀過(guò)后的標(biāo)準(zhǔn)品應(yīng)丟棄,不可保存。           2. 實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中,密封保存,以免變質(zhì)。                                                3. 不用的其它試劑應(yīng)包裝好或蓋好。不同批號(hào)的試劑不要混用。保質(zhì)前使用。                            4. 使用一次性的吸頭以免交叉污染,吸取終止液和底物A、B液時(shí),避免使用帶金屬部分的加樣器。   5. 使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。                    6. 洗滌酶標(biāo)板時(shí)應(yīng)充分拍干,不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。                                   7. 底物A應(yīng)揮發(fā),避免長(zhǎng)時(shí)間打開(kāi)蓋子。底物B對(duì)光敏感,避免長(zhǎng)時(shí)間暴露于光下。避免用手接觸,有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。                                                                                                     8. 加入試劑的順序應(yīng)一致,以保證所有反應(yīng)板孔溫育的時(shí)間一樣。                                                9. 按照說(shuō)明書(shū)中標(biāo)明的時(shí)間、加液的量及順序進(jìn)行溫育操作。

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